BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0128.p (326 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2598 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 299 7e-81 PA2600 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 259 6e-69 PA2356 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 156 7e-38 mll4558 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 156 7e-38 BS_ygcA [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 147 3e-35 >PA2598 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 360 Score = 299 bits (765), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 152/313 (48%), Positives = 208/313 (66%), Gaps = 2/313 (0%) Query: 1 MPVEFIGFITNNNASETIVRSGPVLDPPYIETVAKAHELAGFDRALLAFHSTTPDALQVA 60 M ++F+G I + ASET GP+LD PYI A+AHE AGFDR L+ + S PD VA Sbjct: 1 MSIQFLGMIGHRLASETHAPVGPILDKPYIAAFARAHEEAGFDRILVGYWSDQPDGFLVA 60 Query: 61 QHVLTITKRLKVMIAQRPGFTAPTLLARQLATLDQFYGGRVSLHVITGGNAIELRQDGNT 120 T R+ +++A RPGF APTL AR+LATL+ GR+++H+I+GG+ + R+DG+ Sbjct: 61 AAAGLATSRIGLLLAHRPGFVAPTLAARKLATLEHLLDGRLAVHIISGGSDADQRKDGDY 120 Query: 121 LDDKDERYARTSEFLDVVRLEWTSEKPFNYSGKYYNVENGFSQVKPLQKGGIYTFVGGGS 180 L D D+RYAR+ EF+++++ WTS +PF++ G +Y VE+ FS ++P QK I + GG S Sbjct: 121 L-DHDQRYARSDEFIELLKRTWTSTEPFDFHGAHYRVEHAFSAIRPQQKPHIPVYFGGSS 179 Query: 181 DAAVEVAGKHADTFALWGESYAQVRDVTARVRAAAARHGRPTPRFSLSVRPILADTEEKA 240 +AA++VAGK AD F LWGE AQ + + VR A +GR FSLS RPIL TE++A Sbjct: 180 EAALKVAGKQADVFMLWGEPLAQAAETISAVREQARSNGREV-EFSLSFRPILGRTEDEA 238 Query: 241 WAKAEHILERATALQDQTGYRRPNHATDGAKRLLALADQGTRIDKRLWTEIAKLTGANSN 300 WAKAE I A A + G+ + + GA+RLL +QG R+D+RLWT IA+L G N Sbjct: 239 WAKAEAIRASAGARLGEAGFPKGKPQSVGAQRLLKAVEQGERLDERLWTGIARLVGGGHN 298 Query: 301 TTALVGTPEQVAD 313 +TALVGT EQVAD Sbjct: 299 STALVGTAEQVAD 311 >PA2600 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 355 Score = 259 bits (662), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 144/307 (46%), Positives = 192/307 (62%), Gaps = 4/307 (1%) Query: 8 FITNNNASETIVRSGPVLDPPYIETVAKAHELAGFDRALLAFHSTTPDALQVAQHVLTIT 67 IT +SE G +D ++ A+AHE AGFDR L+ + S + V+ V T Sbjct: 1 MITATPSSEVDQPLGEAVDAGFVRRFAQAHEDAGFDRVLVGYFSNAAEGAVVSSFVAAAT 60 Query: 68 KRLKVMIAQRPGFTAPTLLARQLATLDQFYGGRVSLHVITGGNAIELRQDGNTLDDKDER 127 +RL V++A RPG A L ARQLATLDQF GR++L+V++GGN +L +DG+ L D D R Sbjct: 61 RRLGVLLAYRPGVIAAPLAARQLATLDQFSEGRLALNVVSGGNDEDLARDGDYL-DHDRR 119 Query: 128 YARTSEFLDVVRLEWTSEKPFNYSGKYYNVENGFSQVKPLQKGGIYTFVGGGSDAAVEVA 187 YART E+L +R WT+ P ++ G++Y + V+P Q I + G SDAA+EVA Sbjct: 120 YARTDEYLQALRAIWTATGPVDFDGEFYRFQGASPAVRPRQSPHIPIYFSGSSDAAIEVA 179 Query: 188 GKHADTFALWGESYAQVRDVTARVRAAAARHGRPTPRFSLSVRPILADTEEKAWAKAEHI 247 +HADT+ LWGE A V RVRAAA GR RFS+S RPI+ADTEE AW +A + Sbjct: 180 ARHADTYMLWGEPLAAVDGHIRRVRAAAKAQGRDL-RFSVSFRPIVADTEEAAWKRAAEV 238 Query: 248 LERATALQDQTGYRRPNHATD--GAKRLLALADQGTRIDKRLWTEIAKLTGANSNTTALV 305 LE+ + + G +H G++RLLA A+QG +D RLWT +A+LTGA N+TALV Sbjct: 239 LEQVRENRARLGLPLRDHQPQNVGSQRLLAAAEQGEVLDSRLWTGVARLTGARWNSTALV 298 Query: 306 GTPEQVA 312 GTPEQVA Sbjct: 299 GTPEQVA 305 >PA2356 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 381 Score = 156 bits (394), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 110/295 (37%), Positives = 159/295 (53%), Gaps = 19/295 (6%) Query: 28 PYIETVAKAHELAGFDRALLAFHSTTPDALQVAQHVLTITKRLKVMIAQRPGFTAPTLLA 87 PY++ VA+A + G+ L+ + D+ VA + +T+RL+ ++A RPG +PT+ A Sbjct: 28 PYLKQVAQAADSLGYHGVLIPTGRSCEDSWVVASALAPLTERLRFLVAIRPGIVSPTVSA 87 Query: 88 RQLATLDQFYGGRVSLHVITGGNAIELRQDGNTLDDKDERYARTSEFLDVVRLEWTSEKP 147 R ATLD+ GGR+ ++V+TGG+ E R DG L ERY T EFL V R E Sbjct: 88 RMAATLDRLSGGRLLINVVTGGDPDENRGDGIHLGHA-ERYEVTDEFLRVWRRVLQGEA- 145 Query: 148 FNYSGKYYNVENGFSQVKPLQKGGIYTFVGGGSDAAVEVAGKHADTFALWGESYAQVRDV 207 ++ GK+ +VEN + PLQ+ + GG S+AA E+AG+ D + WGE V Sbjct: 146 VDFHGKHIHVENAKALYPPLQRPYPPLYFGGSSEAAHELAGEQVDVYLTWGEPLPAVAAK 205 Query: 208 TARVRAAAARHGRPTPRFSLSVRPILADTEEKAWAKA----EHILERATALQDQTGYRRP 263 A VR AARHGR T +F + + I+ +T E+AW A EHI + A Q+ R Sbjct: 206 IADVRQRAARHGR-TVKFGIRLHVIVRETAEEAWRAADRLIEHISDETIAAAQQSFAR-- 262 Query: 264 NHATDGAKRLLALADQGTRIDK-----RLWTEIAKLTGANSNTTALVGTPEQVAD 313 ++G +R+ AL G R D+ LW + + G TALVG P QVA+ Sbjct: 263 -FDSEGQRRMAAL--HGGRRDRLEIQPNLWAGVGLVRGGAG--TALVGDPRQVAE 312 >mll4558 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 385 Score = 156 bits (394), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 119/330 (36%), Positives = 167/330 (50%), Gaps = 23/330 (6%) Query: 2 PVEFIGFITNNNASETIVRSGPVLDPP---YIETVAKAHELAGFDRALLAFHSTTPDALQ 58 P++F FI + + + S PP Y + +A+A + GF LL D+ Sbjct: 7 PLDFFWFIPTHGDG-SYLGSEEQQRPPEFGYFKQIAQAVDRLGFPGVLLPTGQNCEDSWI 65 Query: 59 VAQHVLTITKRLKVMIAQRPGFTAPTLLARQLATLDQFYGGRVSLHVITGGNAIELRQDG 118 A + T+T++LK ++A RPG T PT ARQ A LD+ GR+ L+V+ GGN EL DG Sbjct: 66 TATGLATLTEKLKFLVALRPGVTLPTFAARQTAALDRLSNGRLLLNVVVGGNPTELAGDG 125 Query: 119 NTLDDKDERYARTSEFLDVVRLEWTSEKPFNYSGKYYNVENGFSQVKPLQKGGIYTFVGG 178 L DERYA+ EFL + R + E+ N+ GKYY VENG + P Q+ F GG Sbjct: 126 VFL-PHDERYAQAHEFLTIWRGLVSGER-VNFDGKYYRVENGRLDLLPSQERPPLYF-GG 182 Query: 179 GSDAAVEVAGKHADTFALWGESYAQVRDVTARVRAAAARHGRPTPRFSLSVRPILADTEE 238 SDA ++A D + WGE A V + A R AA GR RF + + I+ +TE+ Sbjct: 183 SSDAGQDLAADLVDMYLTWGEPPALVAEKLASARKKAALRGRKL-RFGIRLHFIVRETED 241 Query: 239 KAWAKAEHILERATALQDQTGYRRPNHATD--GAKRLLALADQGTRIDK-----RLWTEI 291 +AW A+ ++ T Q + R + D G +R+ L G R D+ LW + Sbjct: 242 EAWRAADRLISHVTDAQIENAQARFLNQMDSVGQRRMAEL--HGGRRDRLVVSPNLWAGV 299 Query: 292 AKLTGANSNTTALVGTPEQVA----DYSAI 317 + G TALVGTPEQV +Y AI Sbjct: 300 GLVRGGAG--TALVGTPEQVTERIREYQAI 327 >BS_ygcA [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 378 Score = 147 bits (372), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 110/322 (34%), Positives = 158/322 (49%), Gaps = 17/322 (5%) Query: 1 MPVEFIGFITNNNASETI--VRSGPVLDPPYIETVAKAHELAGFDRALLAFHSTTPDALQ 58 M +E + FI + + + G D Y + VA+A + G+ LL + D Sbjct: 1 MNMEILWFIPTHGDARYLGSESDGRTADHLYFKQVAQAADRLGYTGVLLPTGRSCEDPWL 60 Query: 59 VAQHVLTITKRLKVMIAQRPGFTAPTLLARQLATLDQFYGGRVSLHVITGGNAIELRQDG 118 A + TK LK ++A RPG P+L AR +TLD+ GR+ ++V+ GG+ EL DG Sbjct: 61 TASALAGETKDLKFLVAVRPGLMQPSLAARMTSTLDRISDGRLLINVVAGGDPYELAGDG 120 Query: 119 NTLDDKDERYARTSEFLDVVRLEWTSEKPFNYSGKYYNVENGFSQVKPLQKGGIYTFVGG 178 + DERY T EFL V R E +Y GK+ VEN P Q+ + GG Sbjct: 121 LFI-SHDERYEATDEFLTVWRRLLQGET-VSYEGKHIKVENSNLLFPPQQEPHPPIYFGG 178 Query: 179 GSDAAVEVAGKHADTFALWGESYAQVRDVTARVRAAAARHGRPTPRFSLSVRPILADTEE 238 S A +E A KH D + WGE QV++ RV+ AA+ GR RF + + I +TE+ Sbjct: 179 SSQAGIEAAAKHTDVYLTWGEPPEQVKEKIERVKKQAAKEGRSV-RFGIRLHVIARETEQ 237 Query: 239 KAWAKAEHI---LERATALQDQTGYRRPNHATDGAKRLLALADQGTR----IDKRLWTEI 291 +AW AE + L+ T + Q R + + G +R +A+ QG R I LW I Sbjct: 238 EAWEAAERLISHLDDDTIAKAQAALSR--YDSSGQQR-MAVLHQGDRTKLEISPNLWAGI 294 Query: 292 AKLTGANSNTTALVGTPEQVAD 313 + G TALVG P+ +AD Sbjct: 295 GLVRGGAG--TALVGDPQTIAD 314