BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0054.p (671 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs3344 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubuni... 345 3e-94 hyfB [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit N... 344 3e-94 ZhyfB [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit ... 342 2e-93 MA4368 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit... 289 1e-77 Rv0083 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit... 257 8e-68 >ECs3344 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Ecs Escherichia coli O157:H7 Length = 672 Score = 345 bits (884), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 230/603 (38%), Positives = 336/603 (55%), Gaps = 16/603 (2%) Query: 77 RLDALAAFFLVVVNLGGAAASLYGLGY-GSHESAPQRILPFF-PAFLAGMNLVVLADDAF 134 R+D+L+AF ++V++L SLY L Y +E + FF F+A M +++ D+AF Sbjct: 76 RMDSLSAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNIFIASMVALLVMDNAF 135 Query: 135 TYLLCWEFMSLVSWALVMAHHRDADNARAGYVYLVMASFGTLALLLAFGLLAGAGGSYSF 194 +++ +E MSL SW LV+A +D + AG +Y +A G++ +++AF L+ GS F Sbjct: 136 WFIVLFEMMSLSSWFLVIAR-QDKTSINAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDF 194 Query: 195 AAIRAARHTPSEAALVLALMLLGAGSKAGLIPLHVWLPLAHPAAPSHVSALMSGVMTKVA 254 A+ R +P A+ V L G G+KAG++PLH WLP AHPAAPSH SALMSGVM K+ Sbjct: 195 ASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIG 254 Query: 255 IYGFIRVVFDLL---GPPSWSAGVVVLILGGATAVMGILYALMEKDLKRLLAYSTIENIG 311 I+G ++V DLL G P W G++V+ +G +A++G+LYAL E+D+KRLLA+ST+EN+G Sbjct: 255 IFGILKVAMDLLAQTGLPLWW-GILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVG 313 Query: 312 VIFASLGLALAFQANGLGLAAALALTAALFHVLNHSFFKSLLFFGAGAVLTSTGERDMEK 371 +I ++G+A+ + L + L ALFH+LNH+ FK LLF GAGA+++ DMEK Sbjct: 314 IILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEK 373 Query: 372 LGGLIHRMPLTSFVFLVGCVAISALPPFNGFVSEWLMFQAVLQSPDLPQWLLKIMVPAVG 431 +G L RMP T+ L+GC+AISALPP NGF+SEW +Q++ + L++ P Sbjct: 374 MGALAKRMPWTAAACLIGCLAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAM 433 Query: 432 ALLALAAALAAACFVKAFGITFLGRARSRAAEAAAEVDRYSLAAMFSLAVLCFLAGILPG 491 +LA+ LA CFVK +GITF G RS AE A EV + AM LA LC L + Sbjct: 434 VMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSAS 493 Query: 492 LVIDALSPVAQAVIGHRMPLQAEDPWLSIVPIAESRSSYNGLLVFAFITAAASLAVYFIH 551 + + +A A P +++VP LL+ + +Y++ Sbjct: 494 WLAPKIMHIAHAFT--NTPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLW 551 Query: 552 RFASRAI--RRGPAWGCGFSDPTPAAQYSGVSFVQPIRRVFGTLVFQAREHVD--MPAPG 607 + RA R G AW CG+ A SG +QP+R VF L F+ R+ +D + Sbjct: 552 CRSRRAAFRRTGDAWACGYGWENAMAP-SGNGVMQPLRVVFSAL-FRLRQQLDPTLRLNK 609 Query: 608 ELRPARLRVDMHDLVW-ERLYTPVTELVWFFADRLNQLQFLTIRRYLSLVFATLVTLLLA 666 L R + W ER+ P+ A + LQ R Y V A LV LL+A Sbjct: 610 GLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIA 669 Query: 667 LAI 669 +A+ Sbjct: 670 IAV 672 >hyfB [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Eco Escherichia coli K12 Length = 672 Score = 344 bits (883), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 229/603 (37%), Positives = 336/603 (55%), Gaps = 16/603 (2%) Query: 77 RLDALAAFFLVVVNLGGAAASLYGLGY-GSHESAPQRILPFF-PAFLAGMNLVVLADDAF 134 R+D+L+AF ++V++L SLY L Y +E + FF F+A M +++ D+AF Sbjct: 76 RMDSLSAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNIFIASMVALLVMDNAF 135 Query: 135 TYLLCWEFMSLVSWALVMAHHRDADNARAGYVYLVMASFGTLALLLAFGLLAGAGGSYSF 194 +++ +E MSL SW LV+A +D + AG +Y +A G++ +++AF L+ GS F Sbjct: 136 WFIVLFEMMSLSSWFLVIAR-QDKTSINAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDF 194 Query: 195 AAIRAARHTPSEAALVLALMLLGAGSKAGLIPLHVWLPLAHPAAPSHVSALMSGVMTKVA 254 A+ R +P A+ V L G G+KAG++PLH WLP AHPAAPSH SALMSGVM K+ Sbjct: 195 ASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIG 254 Query: 255 IYGFIRVVFDLL---GPPSWSAGVVVLILGGATAVMGILYALMEKDLKRLLAYSTIENIG 311 I+G ++V DLL G P W G++V+ +G +A++G+LYAL E+D+KRLLA+ST+EN+G Sbjct: 255 IFGILKVAMDLLAQTGLPLWW-GILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVG 313 Query: 312 VIFASLGLALAFQANGLGLAAALALTAALFHVLNHSFFKSLLFFGAGAVLTSTGERDMEK 371 +I ++G+A+ + L + L ALFH+LNH+ FK LLF GAGA+++ DMEK Sbjct: 314 IILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEK 373 Query: 372 LGGLIHRMPLTSFVFLVGCVAISALPPFNGFVSEWLMFQAVLQSPDLPQWLLKIMVPAVG 431 +G L RMP T+ L+GC+AISA+PP NGF+SEW +Q++ + L++ P Sbjct: 374 MGALAKRMPWTAAACLIGCLAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAM 433 Query: 432 ALLALAAALAAACFVKAFGITFLGRARSRAAEAAAEVDRYSLAAMFSLAVLCFLAGILPG 491 +LA+ LA CFVK +GITF G RS AE A EV + AM LA LC L + Sbjct: 434 VMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSAS 493 Query: 492 LVIDALSPVAQAVIGHRMPLQAEDPWLSIVPIAESRSSYNGLLVFAFITAAASLAVYFIH 551 + + +A A P +++VP LL+ + +Y++ Sbjct: 494 WLAPKIMHIAHAFT--NTPPATVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLW 551 Query: 552 RFASRAI--RRGPAWGCGFSDPTPAAQYSGVSFVQPIRRVFGTLVFQAREHVD--MPAPG 607 + RA R G AW CG+ A SG +QP+R VF L F+ R+ +D + Sbjct: 552 CRSRRAAFRRTGDAWACGYGWENAMAP-SGNGVMQPLRVVFSAL-FRLRQQLDPTLRLNK 609 Query: 608 ELRPARLRVDMHDLVW-ERLYTPVTELVWFFADRLNQLQFLTIRRYLSLVFATLVTLLLA 666 L R + W ER+ P+ A + LQ R Y V A LV LL+A Sbjct: 610 GLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIA 669 Query: 667 LAI 669 +A+ Sbjct: 670 IAV 672 >ZhyfB [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit EcZ Escherichia coli O157:H7 EDL933 Length = 672 Score = 342 bits (876), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 228/603 (37%), Positives = 335/603 (55%), Gaps = 16/603 (2%) Query: 77 RLDALAAFFLVVVNLGGAAASLYGLGY-GSHESAPQRILPFF-PAFLAGMNLVVLADDAF 134 R+D+L+AF ++V++L SLY L Y +Z + FF F+ M +++ D+AF Sbjct: 76 RMDSLSAFMVLVISLLVVVCSLYSLTYMREYZGKGAAAMGFFMNIFIXSMVALLVMDNAF 135 Query: 135 TYLLCWEFMSLVSWALVMAHHRDADNARAGYVYLVMASFGTLALLLAFGLLAGAGGSYSF 194 +++ +E MSL SW LV+A +D + AG +Y +A G++ +++AF L+ GS F Sbjct: 136 WFIVLFEMMSLSSWFLVIAR-QDKTSINAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDF 194 Query: 195 AAIRAARHTPSEAALVLALMLLGAGSKAGLIPLHVWLPLAHPAAPSHVSALMSGVMTKVA 254 A+ R +P A+ V L G G+KAG++PLH WLP AHPAAPSH SALMSGVM K+ Sbjct: 195 ASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIG 254 Query: 255 IYGFIRVVFDLL---GPPSWSAGVVVLILGGATAVMGILYALMEKDLKRLLAYSTIENIG 311 I+G ++V DLL G P W G++V+ +G +A++G+LYAL E+D+KRLLA+ST+EN+G Sbjct: 255 IFGILKVAMDLLAQTGLPLWW-GILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVG 313 Query: 312 VIFASLGLALAFQANGLGLAAALALTAALFHVLNHSFFKSLLFFGAGAVLTSTGERDMEK 371 +I ++G+A+ + L + L ALFH+LNH+ FK LLF GAGA+++ DMEK Sbjct: 314 IILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEK 373 Query: 372 LGGLIHRMPLTSFVFLVGCVAISALPPFNGFVSEWLMFQAVLQSPDLPQWLLKIMVPAVG 431 +G L RMP T+ L+GC+AISALPP NGF+SEW +Q++ + L++ P Sbjct: 374 MGALAKRMPWTAAACLIGCLAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAM 433 Query: 432 ALLALAAALAAACFVKAFGITFLGRARSRAAEAAAEVDRYSLAAMFSLAVLCFLAGILPG 491 +LA+ LA CFVK +GITF G RS AE A EV + AM LA LC L + Sbjct: 434 VMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSAS 493 Query: 492 LVIDALSPVAQAVIGHRMPLQAEDPWLSIVP-IAESRSSYNGLLVFAFITAAASLAVYFI 550 + + +A A P +++VP ++ + + LL+ + Sbjct: 494 WLAPKIMHIAHAFT--NTPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLXPGLXWLW 551 Query: 551 HRFASRAIRR-GPAWGCGFSDPTPAAQYSGVSFVQPIRRVFGTLVFQAREHVD--MPAPG 607 R A RR G AW CG+ A SG +QP+R VF L F+ R+ +D + Sbjct: 552 CRSRRAAFRRTGDAWACGYGWENAMAP-SGNGVMQPLRVVFSAL-FRLRQQLDPTLRLNK 609 Query: 608 ELRPARLRVDMHDLVW-ERLYTPVTELVWFFADRLNQLQFLTIRRYLSLVFATLVTLLLA 666 L R + W ER+ P+ A + LQ R Y V A LV LL+A Sbjct: 610 GLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIA 669 Query: 667 LAI 669 +A+ Sbjct: 670 IAV 672 >MA4368 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Mac Methanosarcina acetivorans str.C2A Length = 648 Score = 289 bits (740), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 228/668 (34%), Positives = 334/668 (50%), Gaps = 50/668 (7%) Query: 12 VAALLGTTALAIATSRSRQSTSIVYGATFAISLI--ALAGAVISLISGTGATELALPIG- 68 +AAL+ T L + RS+ + +G + S++ AG V+ TG + IG Sbjct: 13 IAALVSGTVLPL-LYRSKNTRKTAFGLSLISSILLLVFAGTVLY----TGKEPVFPAIGF 67 Query: 69 LPWLGSHFRLDALAAFFLVVVNLGGAAASLYGLGYGSH---ESAPQRILPFFPAFLAGMN 125 LP L D L+A F++++ S+Y + Y H E F+ M Sbjct: 68 LPGLDFTLAADRLSAGFILLIAAVVPGVSIYSVEYVEHAKSEDGKNLQAALTNLFILAML 127 Query: 126 LVVLADDAFTYLLCWEFMSLVSWALVMAHHRDADNARAGYVYLVMASFGTLALLLAFGLL 185 +VVLA + +L+ WE MSL S LV+ ++ +N +AG+ Y VM + T L L F L Sbjct: 128 MVVLAGNMVGFLIFWEIMSLSSLLLVLHNYSSEENKKAGFFYFVMTNISTAFLFLGFISL 187 Query: 186 AGAGGSYSFAAIRAARHTPSEAALVLALMLLGAGSKAGLIPLHVWLPLAHPAAPSHVSAL 245 GS F + + E L + +G G KAGL+P H WLP AHPAAPS VSAL Sbjct: 188 FRLTGSTEFGPLE---YAAGELTLPFLSLFVGFGVKAGLVPFHKWLPYAHPAAPSSVSAL 244 Query: 246 MSGVMTKVAIYGFIRVVFDLLGPPSWSAGVVVLILGGATAVMGILYALMEKDLKRLLAYS 305 MSGVM KVA+YGF+R + + P W GV++L G +A+ G++YAL E D+KRLLAYS Sbjct: 245 MSGVMLKVAVYGFLRFLLSISTPELW-WGVLILTAGSLSALFGVIYALKENDIKRLLAYS 303 Query: 306 TIENIGVIFASLGLALAFQANGLGLAAALALTAALFHVLNHSFFKSLLFFGAGAVLTSTG 365 +IENIG+IF +GL + F GL A L+L A FH NH+ FKSLLF AG+V+ +TG Sbjct: 304 SIENIGIIFTGIGLYVIFGVEGLETLALLSLVGACFHSFNHALFKSLLFLCAGSVVHATG 363 Query: 366 ERDMEKLGGLIHRMPLTSFVFLVGCVAISALPPFNGFVSEWLMFQAVLQSPDLPQWLLKI 425 R +E LGGL+ MP TS +F++G V+I+ALPP NGF E L++QA QS + LL + Sbjct: 364 TRKIEALGGLVKSMPATSALFMIGSVSIAALPPTNGFAGELLLYQAFFQSFAVTDPLLTV 423 Query: 426 MVPAVGALLALAAALAAACFVKAFGITFLGRARSRAAEAAAEVDRYSLAAMFSLAVLCFL 485 + + AL ALAAA FVK FGIT L RS + A EV + L A C L Sbjct: 424 FLIIALSSFALTGALAAALFVKLFGITCLAMPRSEKSRLAEEVRKPMLIGPAVPAAFCIL 483 Query: 486 AGILPGLVIDALSPVAQAVIGHRMPLQAEDPWLSIVPIAESRSSYNGLLVFAFITAAASL 545 G+ ++ +A + + LL+ F+ Sbjct: 484 GGLFSK---------------------------QLLSLAGWKIEFPDLLLLGFLLGLIYA 516 Query: 546 AVYFIHRFASRAI---RRGPAWGCGFSDPTPAAQYSGVSFVQPI----RRVFGTLVFQAR 598 A++ + R ++ R W CG P +YS F +P+ + V+ T + Sbjct: 517 ALFVLIRTRTKETNPSRISETWRCGIPTVAPYMEYSSAGFSEPLVMIFKSVYRTKIVHRA 576 Query: 599 EHVDMPAPGELRPARLRVDMHDLVWERLYTPVTELVWFFADRLNQLQFLTIRRYLSLVFA 658 E++D ++ + + + E LY P + F+ +++LQ + Y+ F Sbjct: 577 EYLDR-QESLVKNGNVEIRLVRFFEEYLYIPPARAIEGFSKYVSKLQNGKLDSYVLYAFL 635 Query: 659 TLVTLLLA 666 ++ L +A Sbjct: 636 AVIVLTIA 643 >Rv0083 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Mtu Mycobacterium tuberculosis H37Rv Length = 640 Score = 257 bits (656), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 182/426 (42%), Positives = 237/426 (55%), Gaps = 4/426 (0%) Query: 69 LPWLGSHFRLDALAAFFLVVVNLGGAAASLYGLGYGSHESAPQRILPFFPAFLAGMNLVV 128 LP G H LD L FF+ + A Y +GY E + + P F+A M LV Sbjct: 40 LPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHLGRVPMAVVPLFVAAMLLVP 99 Query: 129 LADDAFTYLLCWEFMSLVSWALVMAHHRDADNARAGYVYLVMASFGTLALLLAFGLLAGA 188 A T+LL WE M++ S LV++ H AG Y VM G +A+L+ +LA A Sbjct: 100 AAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAA 159 Query: 189 GGSYSFAAIRAARHTPSEAALVLALMLLGAGSKAGLIPLHVWLPLAHPAAPSHVSALMSG 248 GGS FA + A A V L L+G GSKAGL+PLH WLP AHP APS VSALMS Sbjct: 160 GGSDRFAGLGAV--CDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSA 217 Query: 249 VMTKVAIYGFIRVVFDLLGP-PSWSAGVVVLILGGATAVMGILYALMEKDLKRLLAYSTI 307 M + IYG +R LLGP P W G+ +L +GG +A+ G+L A + DLKRLLAYST Sbjct: 218 AMVNLGIYGIVRFDLQLLGPGPRWW-GLALLAVGGTSALYGVLQASVAADLKRLLAYSTT 276 Query: 308 ENIGVIFASLGLALAFQANGLGLAAALALTAALFHVLNHSFFKSLLFFGAGAVLTSTGER 367 EN+G+I +LG A F G A++A AA+ H++ H+ FKSL F AG+VL +TG R Sbjct: 277 ENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKSLAFMAAGSVLAATGLR 336 Query: 368 DMEKLGGLIHRMPLTSFVFLVGCVAISALPPFNGFVSEWLMFQAVLQSPDLPQWLLKIMV 427 D++ LGGL RMP T+ F V + LP GFVSEWL+ Q+++ + ++ + Sbjct: 337 DLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTT 396 Query: 428 PAVGALLALAAALAAACFVKAFGITFLGRARSRAAEAAAEVDRYSLAAMFSLAVLCFLAG 487 P ++ALA L+ A KAFGI FL R RS AEAA E A M A C + Sbjct: 397 PLAVGVVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLA 456 Query: 488 ILPGLV 493 + P LV Sbjct: 457 VAPLLV 462