BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0051.p (482 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs3348 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubuni... 301 3e-81 ZhyfF [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit ... 297 4e-80 hyfF [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit N... 295 1e-79 Rv0086 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit... 215 2e-55 MT0093 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit... 215 2e-55 >ECs3348 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Ecs Escherichia coli O157:H7 Length = 526 Score = 301 bits (770), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 177/457 (38%), Positives = 279/457 (61%), Gaps = 12/457 (2%) Query: 27 GYRLTAGLNIVASLG-TLLAALSLLVVERPQP-------GQYLLIDDLNIVFIVLNTFVG 78 G+ T + ++ SLG TLL L+L VV+ G +L ID L +F+ + +G Sbjct: 28 GHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIG 87 Query: 79 FTTSVFSASYIAHELETGRLTPGYLRFYHAMYQVMMFGMNLAFVSNNIGLIWVAVEVATL 138 F T V+S Y+ HE+E G L+P L Y+ + + +F M L SNN+ ++W A+E TL Sbjct: 88 FLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTL 147 Query: 139 TTVLMVGIYRTHAALEAAWKYFILGSVGIALALFGTILVYMAALPVVGEGQDAMVWTVLI 198 ++ +VGIY ++LEAAWKY I+ +VG+A LFGT+LVY A V+ + + A+ W+ ++ Sbjct: 148 SSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVL 207 Query: 199 SRAATFDPALLNVAFIFLLLGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALY 258 +++ DP L+ +AF+F+L+G+GTK GL P+HAWLPDAH+E P+P+SA+LS +LLN AL Sbjct: 208 KQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALL 267 Query: 259 AVLRFKILLAANPDAISPGPLMVTMGLTSLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVF 318 ++R+ I++ + P L++ G+ S+ AAF + +RDIKRL AYSS+E+MG++ Sbjct: 268 VLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAV 327 Query: 319 AFGMGGPLANFAGLLHMVMHSLTKSGIFYAVGHIAQVKGTQRIANIRGLTESHPALGWSL 378 A G+GGPL FA LLH + HSL K+ +F G++ GT+ + + G+ + P Sbjct: 328 ALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLF 387 Query: 379 VIGVVAIAGLPPLGIFMSEFLIVSSTFARQPLLAIPLVFGLLLAFGALILRLTS-LAFGE 437 G +A+AG+PP IF+SEF+ V++ AR LL I L+ LL A ++R+ + + + Sbjct: 388 GGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAK 447 Query: 438 PRGNAAPADASYL---PMFAHFALVLTAGIYLPGPLV 471 P D +L PM ++L G ++P P++ Sbjct: 448 PPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVI 484 >ZhyfF [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit EcZ Escherichia coli O157:H7 EDL933 Length = 526 Score = 297 bits (760), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 176/453 (38%), Positives = 274/453 (60%), Gaps = 12/453 (2%) Query: 31 TAGLNIVASLG-TLLAALSLLVVERPQP-------GQYLLIDDLNIVFIVLNTFVGFTTS 82 T + ++ SLG TLL L+L VV+ G +L ID L +F+ + +GF T Sbjct: 32 TRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTG 91 Query: 83 VFSASYIAHELETGRLTPGYLRFYHAMYQVMMFGMNLAFVSNNIGLIWVAVEVATLTTVL 142 V+S Y+ HE+E G L+P L Y+ + + +F M L SNN+ ++W A+E TL++ Sbjct: 92 VYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAF 151 Query: 143 MVGIYRTHAALEAAWKYFILGSVGIALALFGTILVYMAALPVVGEGQDAMVWTVLISRAA 202 +VGIY ++LEAAWKY I+ +VG+A LFGT+LVY A V+ + + A+ W+ ++ +++ Sbjct: 152 LVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSS 211 Query: 203 TFDPALLNVAFIFLLLGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYAVLR 262 DP L+ +AF+F+L+G+GTK GL P+HAWLPDAH+E P+P+SA+LS +LLN AL ++R Sbjct: 212 LLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIR 271 Query: 263 FKILLAANPDAISPGPLMVTMGLTSLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVFAFGM 322 + I++ + P L++ G+ S+ AAF + +RDIKRL AYSS+E+MG++ A G+ Sbjct: 272 YYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGI 331 Query: 323 GGPLANFAGLLHMVMHSLTKSGIFYAVGHIAQVKGTQRIANIRGLTESHPALGWSLVIGV 382 GGPL FA LLH + HSL K+ +F G++ GT+ + + G+ + P G Sbjct: 332 GGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGA 391 Query: 383 VAIAGLPPLGIFMSEFLIVSSTFARQPLLAIPLVFGLLLAFGA-LILRLTSLAFGEPRGN 441 +A+AG+PP IF+SEF+ V++ AR LL I L+ LL A L+ + +P Sbjct: 392 LALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVXMAARVLMAKPPQA 451 Query: 442 AAPADASYL---PMFAHFALVLTAGIYLPGPLV 471 D +L PM ++L G ++P P++ Sbjct: 452 VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVI 484 >hyfF [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Eco Escherichia coli K12 Length = 526 Score = 295 bits (756), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 173/453 (38%), Positives = 276/453 (60%), Gaps = 12/453 (2%) Query: 31 TAGLNIVASLG-TLLAALSLLVVERPQP-------GQYLLIDDLNIVFIVLNTFVGFTTS 82 T + ++ SLG TLL L+L VV+ G +L ID L +F+ + +GF T Sbjct: 32 TRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTG 91 Query: 83 VFSASYIAHELETGRLTPGYLRFYHAMYQVMMFGMNLAFVSNNIGLIWVAVEVATLTTVL 142 ++S Y+ HE+ G L+P L Y+ + + +F M L SNN+ ++W A+E TL++ Sbjct: 92 IYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAF 151 Query: 143 MVGIYRTHAALEAAWKYFILGSVGIALALFGTILVYMAALPVVGEGQDAMVWTVLISRAA 202 +VGIY ++LEAAWKY I+ +VG+A LFGT+LVY A V+ + + A+ W+ ++ +++ Sbjct: 152 LVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSS 211 Query: 203 TFDPALLNVAFIFLLLGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYAVLR 262 DP L+ +AF+FLL+G+GTK GL P+HAWLPDAH+E P+P+SA+LS +LLN AL ++R Sbjct: 212 LLDPTLMLLAFVFLLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIR 271 Query: 263 FKILLAANPDAISPGPLMVTMGLTSLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVFAFGM 322 + I++ + P L++ G+ S+ AAF + +RDIKRL AYSS+E+MG++ G+ Sbjct: 272 YYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVELGI 331 Query: 323 GGPLANFAGLLHMVMHSLTKSGIFYAVGHIAQVKGTQRIANIRGLTESHPALGWSLVIGV 382 GGPL FA LLH++ HSL K+ +F G++ GT+ + + G+ + P G Sbjct: 332 GGPLGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGA 391 Query: 383 VAIAGLPPLGIFMSEFLIVSSTFARQPLLAIPLVFGLLLAFGALILRLTS-LAFGEPRGN 441 +A+AG+PP IF+SEF+ +++ AR LL I L+ LL A ++R+ + + +P Sbjct: 392 LALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA 451 Query: 442 AAPADASYL---PMFAHFALVLTAGIYLPGPLV 471 D +L PM ++L G ++P P++ Sbjct: 452 VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVI 484 >Rv0086 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit Mtu Mycobacterium tuberculosis H37Rv Length = 488 Score = 215 bits (547), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 164/429 (38%), Positives = 231/429 (53%), Gaps = 12/429 (2%) Query: 58 GQYLLIDDLNIVFIVLNTFVGFTTSVFSASYIAHELETGRLTPGYLRFYHAMYQVMMFGM 117 G L D L +V +V+ VG + S YI EL G + R Y + + M Sbjct: 59 GGLLRADALTVVMLVVIGIVGTLATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAM 118 Query: 118 NLAFVSNNIGLIWVAVEVATLTTVLMVGIYRTHAALEAAWKYFILGSVGIALALFGTILV 177 LA +NNIG+IWVA+E T+ T +VG RT ALEA WKY ++ SVGIA+A GT+L+ Sbjct: 119 VLAVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKYVVICSVGIAVAFLGTVLL 178 Query: 178 YMAALPVVGEGQDAMVWTVLISRAATFDPALLNVAFIFLLLGYGTKVGLAPLHAWLPDAH 237 Y AA A+ +L AA DP + +A LL+GYG K GL P H WL DAH Sbjct: 179 YFAARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAH 238 Query: 238 AEGPTPISAVLSGLLLNVALYAVLRFKILLAA--NPDAISPGPLMVTMGLTSLLFAAFML 295 ++ P P+SA++SG+LL VA ++R + +L A P + G L+V GL +LL A ML Sbjct: 239 SQAPAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVV--GLATLLVAVLML 296 Query: 296 YRRRDIKRLFAYSSIEHMGIIVFAFGMGGPLANFAGLLHMVMHSLTKSGIFYAVGHIAQV 355 D+KR+ AYSS+EHMG+I A G LA A LLH++ H + K+ +F A G + Sbjct: 297 TVTGDVKRMLAYSSMEHMGLIAIAAAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAA 356 Query: 356 KGTQRIANIRGLTESHPALGWSLVIGVVAIAGLPPLGIFMSEFLIVSSTFARQPLLAIPL 415 + IA+I G+ +G S +G++ + GLPP +F SE L ++ + A + L + Sbjct: 357 HDSTAIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASE-LAIARSLANERLAWVLG 415 Query: 416 VFGLLLAFG--ALILRLTSLAFGEPRGNAAPADASYLPMFAHFALVLTAGIYLPGPLVVW 473 LL+A G AL + G P AA A A +P A AL++ GI + L + Sbjct: 416 AALLLIAIGFTALARNSGRMLLGTP---AAGAPAITVPATAAAALMV--GIVVSAALGIT 470 Query: 474 FQHVAHLLG 482 +A LLG Sbjct: 471 AGPLADLLG 479 >MT0093 [CP] COG0651 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit MtC Mycobacterium tuberculosis CDC1551 Length = 488 Score = 215 bits (547), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 164/429 (38%), Positives = 231/429 (53%), Gaps = 12/429 (2%) Query: 58 GQYLLIDDLNIVFIVLNTFVGFTTSVFSASYIAHELETGRLTPGYLRFYHAMYQVMMFGM 117 G L D L +V +V+ VG + S YI EL G + R Y + + M Sbjct: 59 GGLLRADALTVVMLVVIGIVGTLATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAM 118 Query: 118 NLAFVSNNIGLIWVAVEVATLTTVLMVGIYRTHAALEAAWKYFILGSVGIALALFGTILV 177 LA +NNIG+IWVA+E T+ T +VG RT ALEA WKY ++ SVGIA+A GT+L+ Sbjct: 119 VLAVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKYVVICSVGIAVAFLGTVLL 178 Query: 178 YMAALPVVGEGQDAMVWTVLISRAATFDPALLNVAFIFLLLGYGTKVGLAPLHAWLPDAH 237 Y AA A+ +L AA DP + +A LL+GYG K GL P H WL DAH Sbjct: 179 YFAARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAH 238 Query: 238 AEGPTPISAVLSGLLLNVALYAVLRFKILLAA--NPDAISPGPLMVTMGLTSLLFAAFML 295 ++ P P+SA++SG+LL VA ++R + +L A P + G L+V GL +LL A ML Sbjct: 239 SQAPAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVV--GLATLLVAVLML 296 Query: 296 YRRRDIKRLFAYSSIEHMGIIVFAFGMGGPLANFAGLLHMVMHSLTKSGIFYAVGHIAQV 355 D+KR+ AYSS+EHMG+I A G LA A LLH++ H + K+ +F A G + Sbjct: 297 TVTGDVKRMLAYSSMEHMGLIAIAAAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAA 356 Query: 356 KGTQRIANIRGLTESHPALGWSLVIGVVAIAGLPPLGIFMSEFLIVSSTFARQPLLAIPL 415 + IA+I G+ +G S +G++ + GLPP +F SE L ++ + A + L + Sbjct: 357 HDSTAIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASE-LAIARSLANERLAWVLG 415 Query: 416 VFGLLLAFG--ALILRLTSLAFGEPRGNAAPADASYLPMFAHFALVLTAGIYLPGPLVVW 473 LL+A G AL + G P AA A A +P A AL++ GI + L + Sbjct: 416 AALLLIAIGFTALARNSGRMLLGTP---AAGAPAITVPATAAAALMV--GIVVSAALGIT 470 Query: 474 FQHVAHLLG 482 +A LLG Sbjct: 471 AGPLADLLG 479