BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0046.p (259 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1166 [Q] COG0412 Dienelactone hydrolase and related enzymes Pa... 198 1e-50 PA1597 [Q] COG0412 Dienelactone hydrolase and related enzymes Pa... 145 1e-34 mll3556 [Q] COG0412 Dienelactone hydrolase and related enzymes M... 83 6e-16 alr4786 [Q] COG0412 Dienelactone hydrolase and related enzymes N... 80 4e-15 aq_1997 [Q] COG0412 Dienelactone hydrolase and related enzymes A... 79 1e-14 >PA1166 [Q] COG0412 Dienelactone hydrolase and related enzymes Pae Pseudomonas aeruginosa Length = 262 Score = 198 bits (503), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 107/239 (44%), Positives = 146/239 (61%), Gaps = 5/239 (2%) Query: 12 SAHAAV--KEEPVTYSDGETTMKGFVVYDDATQAKRPGIVMVHEWWGITPHIHNEARKFA 69 S AA+ +E P +DG T M G+ YDD+ RPG+++VHEWWG+ + AR A Sbjct: 15 SVQAAIQTREMPYRSADG-TRMVGYFAYDDSKPGIRPGVIVVHEWWGLNDYAKRRARDLA 73 Query: 70 EQGYVAFIADMYGDAKTADNPKDAGALSGSVMKNPKVMEQRFNAARERLAEQASVNPQRI 129 E GY A DMYG+ K ++P+DA A + ++ + RF A E L Q +P +I Sbjct: 74 ELGYSALAIDMYGEGKHTEHPQDAMAFMQAATRDADAAKARFLAGLELLKRQPQTDPSQI 133 Query: 130 GAVGYCFGGAVVLNMARTGADLAAVAGFHASLGLNTPAPAPGTVKAKILILNGADDPFVK 189 A+GYCFGG +VL+MAR G LA VA FH +LG TPA + G+VKAKIL+ +G+ D V Sbjct: 134 AAIGYCFGGKIVLDMARQGLPLAGVASFHGALGTATPA-SKGSVKAKILVEHGSADSLVP 192 Query: 190 REQYDALKKDFDAAKADYRIVEYPGAVHAFTNPEATELGKKFNLPLRYDAKADQESKAE 248 + DALK++ AA ADYR+V GA H FTNP+A + K L + YD +AD+ S A+ Sbjct: 193 AKDLDALKQELSAAGADYRVVIQDGAKHGFTNPDA-DAHKGHGLDIGYDRQADRRSWAD 250 >PA1597 [Q] COG0412 Dienelactone hydrolase and related enzymes Pae Pseudomonas aeruginosa Length = 241 Score = 145 bits (366), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 83/232 (35%), Positives = 126/232 (54%), Gaps = 10/232 (4%) Query: 17 VKEEPVTYSDGETTMKGFVVYDDATQAKRPGIVMVHEWWGITPHIHNEARKFAEQGYVAF 76 ++ EPV Y +G +VYD + RPG++M W G++ + AR+ A +G+V Sbjct: 4 IRVEPVAYDIDGQPYEGQLVYDASHAGPRPGLLMAPNWMGVSAAALDIARQVAGRGHVVL 63 Query: 77 IADMYGDAKTADNPKDAGALSGSVMKNPKVMEQRFNAARERLAEQ--ASVNPQRIGAVGY 134 +AD+YG N +AGA + + ++ +R AA L Q A+V+ R A G+ Sbjct: 64 VADLYGRDVRPQNGDEAGAAMMPLKNDRALLRKRMQAALAALRGQALAAVDTTRQAAFGF 123 Query: 135 CFGGAVVLNMARTGADLAAVAGFHASLGLNTPAPAPG-TVKAKILILNGADDPFVKREQY 193 CFGG L +AR GA+L A FH + L+TP PA +K +L+L+GA DP V REQ Sbjct: 124 CFGGCCALELARDGAELKAFVSFHGT--LDTPDPAHARNIKGAVLVLDGASDPLVPREQL 181 Query: 194 DALKKDFDAAKADYRIVEYPGAVHAFTNPEATELGKKFNLPLRYDAKADQES 245 A ++ A D+++ Y GAVH+FT+P A GK + YDA+ + + Sbjct: 182 PAFAREMTDAGVDWQLTSYGGAVHSFTDPNAKLPGK-----MHYDARTSRRA 228 >mll3556 [Q] COG0412 Dienelactone hydrolase and related enzymes Mlo Mesorhizobium loti Length = 244 Score = 83.2 bits (204), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 73/251 (29%), Positives = 106/251 (42%), Gaps = 13/251 (5%) Query: 10 VASAHAAVKEEPVTYSDGETTMKGFVVYDDATQAKRPGIVMVHEWWGITPHIHNEARKFA 69 AS +A +P+ Y D T G +V D A R +V V +W+GI + EAR+FA Sbjct: 4 TASGLSATPGQPLVYRDDGTDFSGRIVMPDG--AVRGSLVYVPDWYGIYDYTLAEARRFA 61 Query: 70 EQGYVAFIADMYGDAKTADNPKDAGALSGSVMKNPKVMEQRFNAARERLAEQASVNPQR- 128 G+ +AD+YG + A S + NP+ +R A + V PQ Sbjct: 62 SLGFAVMVADVYGAGVRITSDAQATQASQFLRDNPRTASRRLQAGVRAFRD---VVPQAP 118 Query: 129 -IGAVGYCFGGAVVLNMARTGADLAAVAGFHASLGLNTPAPAPGTVKAKILILNGADDPF 187 + AVG+ GG VL M + +LG PA ++ I +G D Sbjct: 119 FLAAVGFSLGGFCVLEMLTDAPLVRGAVILSGALG--QPAGDYAAIETPIRFHHGTRDMV 176 Query: 188 VKREQYDALKKDFDAAKADYRIVEYPGAVHAFTNPEATELGKKFNLPLRYDAKADQESKA 247 + + +A D + Y GA HAFTNP G + L YDA+ E+ A Sbjct: 177 ADVARVTKVLAWLEAKGCDCALTLYAGARHAFTNPNLPSGGDGW---LGYDARYAGEADA 233 Query: 248 EAAKLFAANLK 258 A + F +LK Sbjct: 234 -ATESFLLSLK 243 >alr4786 [Q] COG0412 Dienelactone hydrolase and related enzymes Nos Nostoc sp. PCC 7120 Length = 250 Score = 80.5 bits (197), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 62/253 (24%), Positives = 114/253 (45%), Gaps = 19/253 (7%) Query: 12 SAHAAVKEEPVTYSDGETTMKGFVVYDDATQAKRPGIVMVHEWWGITPHIHNEARKFAEQ 71 S +++ V S + M F Y + ++P ++++ E +G+T HI + A + A++ Sbjct: 11 SNKVQIEKTEVEISTPDGKMPAFF-YQPCERGQKPAVILLMEAFGLTSHIQDVATRIAKE 69 Query: 72 GYVAFIADMYGDAKT-----ADNPKDAGALSGSVMKNPKVMEQRFNAARERLAEQASVNP 126 GYV D+Y T + + A A+ + K +E+ AA L Q++V Sbjct: 70 GYVVLTPDLYYRELTNNKFGYEEVEQAMAMMYR-LDFGKPIEEDIRAAIAYLKSQSNVFS 128 Query: 127 QRIGAVGYCFGGAV-VLNMARTGADLAAVAGFHASLGLNTPAPAPGTVKAKILILNGADD 185 ++IG G+C GG + L+ + ++AAVA F+ + L+ A + I + G D Sbjct: 129 EKIGVTGFCLGGGLSFLSACKFSDEIAAVASFYGMV-LDDWIEAVTNISVPIYLFYGGID 187 Query: 186 PFVKREQYDALKKDFDAAKADYRIVEYPGAVHAFTNPEATELGKKFNLPLRYDAKADQES 245 F+ E+ ++ F +Y + YP A H F E + Y+ A ++S Sbjct: 188 SFIPPERVQQIETRFQELSKEYTLKVYPDADHGFFCHERSS----------YNRLAAEDS 237 Query: 246 KAEAAKLFAANLK 258 E + F +L+ Sbjct: 238 WNELTQFFHRHLQ 250 >aq_1997 [Q] COG0412 Dienelactone hydrolase and related enzymes Aae Aquifex aeolicus Length = 231 Score = 78.6 bits (192), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 58/217 (26%), Positives = 94/217 (43%), Gaps = 15/217 (6%) Query: 46 PGIVMVHEWWGITPHIHNE---ARKFAEQGYVAFIADMYGDAKTADNPKDAGALSGSVM- 101 P +++ HEWWG+ + N K A++G+VAF D Y + ADNP DAG L + Sbjct: 26 PAVLVFHEWWGLESPLSNIKEICDKLADEGFVAFAPDFY-KGQYADNPDDAGKLMTEMFE 84 Query: 102 KNPKVMEQRFNAARERLAEQASVNPQRIGAVGYCFGGAVVLNMARTGADLAAVAGFHASL 161 K +++ F A+ E + E P+++G G+C GG + + A ++ + L Sbjct: 85 KRMDEVDRIFQASVEFVKECRYTYPKKVGITGFCCGGTLAMYFAAKFPEMVDASLPFYGL 144 Query: 162 GLNTPAPAPGTVKAKILILNGADDPFVKREQYDALKKDFDAAKADYRIVEYPGAVHAFTN 221 T A +K I + D FV ++ + K D ++ + G HAF N Sbjct: 145 PQLTQINAE-NIKVPIFFILAEKDEFVNNDEVIDIAKTVWKNGVDVQVKVFSGVTHAFLN 203 Query: 222 PEATELGKKFNLPLRYDAKADQESKAEAAKLFAANLK 258 + ++ YD K E+ A F LK Sbjct: 204 EKREDV---------YDPKRACEAWELAVNFFKTYLK 231