BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0021.p (551 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0027 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 699 0.0 ML2667 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP famil... 476 e-134 CAC3329 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 188 2e-47 CAC0685 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP fami... 170 9e-42 DR1709 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP famil... 88 5e-17 >RSp0027 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Rso Ralstonia solanacearum Length = 547 Score = 699 bits (1803), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/542 (69%), Positives = 440/542 (81%), Gaps = 9/542 (1%) Query: 8 QTAILDSAHAGDIHGALGSIARDDTAPRATLPARLKTLLAIVGPGLIVMVGDNDAGAFGT 67 + A LD AH GDI GALG+IA D PR ARL+TLLAI+GPGLIVMVGDNDAGAFGT Sbjct: 13 RRATLDDAHVGDIRGALGTIAPHDIGPRRGWRARLRTLLAILGPGLIVMVGDNDAGAFGT 72 Query: 68 YTQAGQNYGTTLLWTLLLLIPVLYVNQEMVVRLGAVTGVGHARLIFERFGKFWGAFSVID 127 Y QAGQNYGTTLLWTLLLLIPVLYVNQEMV+RLGAVT VGHARLIF RFG+FWG+FSVID Sbjct: 73 YAQAGQNYGTTLLWTLLLLIPVLYVNQEMVLRLGAVTRVGHARLIFARFGRFWGSFSVID 132 Query: 128 LLVLNALTIVTEFIGITLALHFLGVSQFWGVLASAVIVMLAVSTGDFRRFERFGIVLVAG 187 L ++NALT+VTEFIGI+LAL +LG+ + WGV SA +V+LA +TGDFRRFERF + LV G Sbjct: 133 LFLVNALTLVTEFIGISLALAYLGIPRHWGVCVSAALVLLAAATGDFRRFERFALALVVG 192 Query: 188 SLLLVPVILMVHPPIGQIAADFFVPKMPQDAKLGEVMLLIVAIVGTTVAPWQLFFQQSYI 247 SL L+PV+ M HPP+GQ+A DFFVP +P A L EV+LLI+AIVGTTVAPWQLFFQQSY+ Sbjct: 193 SLALIPVLAMAHPPLGQMARDFFVPALPAGAPLSEVVLLIIAIVGTTVAPWQLFFQQSYV 252 Query: 248 VDKRITPRFIRYERMDLCIGIVLVVVGAVAMISFCAAVFAGKPEFGNYTDALGTAVGLET 307 +DKRITPRFIRYER DL +GIVL VVGAVAM++F A F G PEFG YTDALGTAVGLE Sbjct: 253 IDKRITPRFIRYERADLWLGIVLAVVGAVAMMAFSAQAFHGTPEFGRYTDALGTAVGLER 312 Query: 308 YAGRLPAVLFALALLDACIIGAAAVSLATAYAIGDVFAVKHSLHRKPWDAKGFYSVYCGL 367 +AGR +LFA+ALLDA IIGA AVSL+TAYAIGDV AV+HSLHRKP +A+ FY+VY GL Sbjct: 313 HAGRWAGILFAIALLDASIIGACAVSLSTAYAIGDVLAVRHSLHRKPTEARCFYAVYFGL 372 Query: 368 IVLAAVLVLTPGTPLGLLTNAVQTLAGVLLPSATVFLLLLCNDRHILGPWVNSTKLNLFT 427 +LAA LVLTPG PLGLLT+AVQ+LAGVLLPSATVFLLLLCND+ +LGPW NS NLFT Sbjct: 373 TLLAAGLVLTPGVPLGLLTSAVQSLAGVLLPSATVFLLLLCNDKAVLGPWTNSRLTNLFT 432 Query: 428 GAVVAGLVMLSVILTAAVLFPDLGEAWIIGILVGGSLLALAVTAAV-KFYEMSSRGKAPF 486 G VVA LVMLSVILTAAVLFP +G I+ +L GGSL+A V+ A+ + ++ G A Sbjct: 433 GTVVAVLVMLSVILTAAVLFPGIGMVEIVAVLAGGSLIAAVVSLALWRIERRANHGIA-- 490 Query: 487 RFKRKPSQEPCLIDRDSWRMPPLDRLPPVRLSALSRIWMIVLRGYLVVAAGLVLLRIVQL 546 +R+ S ++DR W MPPL++L RL+ L R WM+VLRGYLV+AAGLVL+RI L Sbjct: 491 --RRRVS----VLDRTRWTMPPLEQLTAARLTPLKRTWMLVLRGYLVIAAGLVLVRIAGL 544 Query: 547 AA 548 A Sbjct: 545 VA 546 >ML2667 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Mle Mycobacterium leprae Length = 618 Score = 476 bits (1226), Expect = e-134, Method: Compositional matrix adjust. Identities = 275/549 (50%), Positives = 359/549 (65%), Gaps = 23/549 (4%) Query: 5 KTTQTAILDSAHAGDIHGALGSIARDDTAPRATLPARLKTLLAIVGPGLIVMVGDNDAGA 64 T+ +++LDSAH GDI GA G I D T + +L+ ++ I GPGLI M+GDNDAG Sbjct: 53 DTSGSSVLDSAHVGDIVGAFGRIGWDVTHASRSRWQQLRLMMVITGPGLIAMIGDNDAGG 112 Query: 65 FGTYTQAGQNYGTTLLWTLLLLIPVLYVNQEMVVRLGAVTGVGHARLIFERFGKFWGAFS 124 TY QAGQ YG LLWTL+LLIPVLYVNQEMV+RLGAVT VGHARLIFERFGKFWGAFS Sbjct: 113 VATYAQAGQKYGMNLLWTLVLLIPVLYVNQEMVLRLGAVTRVGHARLIFERFGKFWGAFS 172 Query: 125 VIDLLVLNALTIVTEFIGITLALHFLGVSQFWGVLASAVIVMLAVSTGDFRRFERFGIVL 184 V DLL+LNALTIVTEFIG++LAL FLG + + +AV++ V+ G FRR+E+ +L Sbjct: 173 VGDLLILNALTIVTEFIGVSLALDFLGCPKTIAIPVAAVLLFAMVAGGSFRRWEQVMFML 232 Query: 185 VAGSLLLVPVILMVHPPIGQIAADFFVPKMP--QDAKLGEVMLLIVAIVGTTVAPWQLFF 242 ++ ++ ++P++L VHP +G + +P +P D+ V+L+I+AIVGTTVAPWQLFF Sbjct: 233 ISVNVAVIPLVLSVHPALGP-SLHGLLPSLPGKPDSPTSVVLLVIMAIVGTTVAPWQLFF 291 Query: 243 QQSYIVDKRITPRFIRYERMDLCIGIVLVVVGAVAMISFCAAVFAGKPEFGNYTDALGTA 302 QQS +VDKRITPR++RY R DL +GIV V+ GA+A++S A AG + GN+TDA A Sbjct: 292 QQSSVVDKRITPRWMRYGRADLVVGIVGVMAGAIALMSATAFGLAGTADSGNFTDAGAVA 351 Query: 303 VGLETYAGRLPAVLFALALLDACIIGAAAVSLATAYAIGDVFAVKHSLHRKPWDAKGFYS 362 L + G VLFA+ LLDA +IGA V LAT YA+GD HSLH K +A FY Sbjct: 352 SHLSDHVGHPIGVLFAIILLDASLIGANVVGLATTYALGDALGKPHSLHWKITEAPLFYG 411 Query: 363 VYCGLIVLAAVLVLTPGTPLGLLTNAVQTLAGVLLPSATVFLLLLCNDRHILGPWVNSTK 422 Y L+ ++AV+ +PG LGL+T VQ LAGVLLPSATVFL+LLCNDR +LGPWVN+ + Sbjct: 412 SYTALLGISAVVAFSPGHILGLITQGVQALAGVLLPSATVFLVLLCNDRPVLGPWVNTAR 471 Query: 423 LNLFTGAVVAGLVMLSVILTAAVLFPDLGEAWIIGILVGGSLLALAVTAA---------- 472 N+F +V LV+LS++LT LFP+L A ++G L G+ L + AA Sbjct: 472 QNVFAWMIVWSLVVLSLMLTVVTLFPNLSTAAMVGGLGAGTALGVVGAAAVIVAGWLAER 531 Query: 473 ------VKFYEMSSRGKAPF----RFKRKPSQEPCLIDRDSWRMPPLDRLPPVRLSALSR 522 + S +A F R R + C R +W MP L L +S + R Sbjct: 532 RGAGGIAQQPSCSGSEQAQFDALARLTRAERRALCDERRANWTMPVLANLDRPVMSPVRR 591 Query: 523 IWMIVLRGY 531 I +++LRGY Sbjct: 592 IGLLMLRGY 600 >CAC3329 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Cac Clostridium acetobutylicum Length = 411 Score = 188 bits (478), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 128/407 (31%), Positives = 207/407 (50%), Gaps = 17/407 (4%) Query: 41 RLKTLLAIVGPGLIVMVGDNDAGAFGTYTQAGQNYGTTLLWTLLLLIPVLYVNQEMVVRL 100 +L +L I+GPGLI + NDAG TY G +YG +LW LLL+ L + QEM R+ Sbjct: 6 KLLFILGIIGPGLITVNAGNDAGGIATYATVGASYGYKMLWGLLLITFSLAIIQEMNARM 65 Query: 101 GAVTGVGHARLIFERFGKFWGAFSVIDLLVLNALTIVTEFIGITLALHFLGVSQFWGVLA 160 VTG G + LI E +G W F++ L + N V +F GI +L G+S++ V Sbjct: 66 AVVTGKGLSDLIRENYGVKWSLFAMCILFIANFGVCVGDFAGIAASLELFGISKYITVPI 125 Query: 161 SAVIVMLAVSTGDFRRFERFGIVLVAGSLLLVPVILMVHPPIGQIAADFFVPKMPQDAKL 220 A +V ++ G + + E+ + + + VHP + P + Sbjct: 126 MAFLVWFVITKGSYEKTEKVFLAFTFVFFTYIITCIKVHPNWNVVMKATITPSLSFTK-- 183 Query: 221 GEVMLLIVAIVGTTVAPWQLFFQQSYIVDKRITPRFIRYERMDLCIGIVLVVVGAV--AM 278 G V+ LI ++GTT+ P+ F+ QS +VDK ++ + +YE++D V +GA+ + Sbjct: 184 GYVLTLI-GMIGTTITPYMQFYLQSSVVDKGLSVKEYKYEKID-------VYLGAIWGDL 235 Query: 279 ISF----CAAVFAGKPEFGNYTDALGTAVGLETYAGRLPAVLFALALLDACIIGAAAVSL 334 +SF C AV K A A+ L+ AG +LFA L A ++ + L Sbjct: 236 VSFFIIVCTAVTLYKAGI-KIDSAEQAAMALKPLAGNYATILFAAGLFGASVLATMVIPL 294 Query: 335 ATAYAIGDVFAVKHSLHRKPWDAKGFYSVYCGLIVLAAVLVLTPGTPLGLLTNAVQTLAG 394 +T YAI + F + L +AK FY ++ +I+ +A LVL P L + Q +AG Sbjct: 295 STTYAICEAFGFESGLDNSFSEAKTFYGIFTFMILFSAALVLVPKLSLVGIMLITQQIAG 354 Query: 395 VLLPSATVFLLLLCNDRHILGPWVNSTKLNLFTGAVVAGLVMLSVIL 441 +L P +F++LL N++ I+G ++N+ N+ VA +++LS+IL Sbjct: 355 ILSPVILIFMVLLINNKDIMGKYINNKLQNVIVYLTVAFIIILSLIL 401 >CAC0685 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Cac Clostridium acetobutylicum Length = 408 Score = 170 bits (430), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 115/388 (29%), Positives = 197/388 (50%), Gaps = 9/388 (2%) Query: 40 ARLKTLLAIVGPGLIVMVGDNDAGAFGTYTQAGQNYGTTLLWTLLLLIPVLYVNQEMVVR 99 +RL + +IVGPGLI NDAG TY+ G YG ++LW L + L V QEM R Sbjct: 6 SRLLFIFSIVGPGLITANAGNDAGGVTTYSVIGSKYGYSMLWGLFAIGIGLAVVQEMNAR 65 Query: 100 LGAVTGVGHARLIFERFGKFWGAFSVIDLLVLNALTIVTEFIGITLALHFLGVSQFWGVL 159 + VTG G + LI ER+G F+++ L++ N + +F GI +L ++++ V Sbjct: 66 MAVVTGKGLSDLIRERYGVKLAFFAMLVLMIANLGVCIADFAGIAASLQLFNINKYISVP 125 Query: 160 ASAVIVMLAVSTGDFRRFERFGIVLVAGSLLLVPVILMVHPPIGQIAADFFVPKMPQDAK 219 A+++ + ++ G++ + E+ ++L V M+ P + + P Sbjct: 126 ILAIMIWIIMTKGNYSKAEKVFLLLTLTFFGYVITAFMLKPNWSNVFSHI---SNPGGTL 182 Query: 220 LGEVMLLIVAIVGTTVAPWQLFFQQSYIVDKRITPRFIRYERMDLCIGIVLVVVGAVAMI 279 L ++ +VGTT+ P+ F+ QS IVDK I+ + YE++D+ +G V ++ A+ I Sbjct: 183 SKNYWLDLIGMVGTTITPYMQFYLQSSIVDKGISLKEYGYEKLDVYLGTVWGILTAL-FI 241 Query: 280 SFCAA--VFAGKPEFGNYTDALGTAVGLETYAGRLPAVLFALALLDACIIGAAAVSLATA 337 + C + +F + DA A L+ AG+ VLF + L A + + L+T+ Sbjct: 242 TICTSETLFKAHMAISSAQDA---AAALKPLAGQYSFVLFGVGLFGASFLACCIIPLSTS 298 Query: 338 YAIGDVFAVKHSLHRKPWDAKGFYSVYCGLIVLAAVLVLTPGTPLGLLTNAVQTLAGVLL 397 YA+ + F + L K +A F+ ++ +I+ + ++VL P L + Q LAGVL Sbjct: 299 YAVCEAFGFESGLDNKIKEAPVFFGLFFFMIITSVIIVLMPNISLISIILFTQQLAGVLC 358 Query: 398 PSATVFLLLLCNDRHILGPWVNSTKLNL 425 P +F++ L ND+ I+G N+ N Sbjct: 359 PVVLIFMIKLVNDKEIMGKHTNNRLQNF 386 >DR1709 [P] COG1914 Mn2+ and Fe2+ transporters of the NRAMP family Dra Deinococcus radiodurans Length = 436 Score = 88.2 bits (217), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 115/411 (27%), Positives = 179/411 (43%), Gaps = 52/411 (12%) Query: 25 GSIARDDTAPRATLPARLKTLLAIVGPGLIVMVGDNDAGAFGTYTQAGQNYGTTLLWTLL 84 G+ R PR ++ +L +GP +I + D G F T + G YG +LLW +L Sbjct: 25 GATLRGTAGPRG-----VRRILPFLGPAVIASIAYMDPGNFATNIEGGARYGYSLLWVIL 79 Query: 85 LLIPVLYVNQEMVVRLGAVTGVGHARLIFERFGKFWGAFSVIDLLVLNALTIVTEFIGIT 144 + V Q + LG +G LI ER+ + F I ++ T + EF+G Sbjct: 80 AANLMAMVIQNLSANLGIASGRNLPELIRERWPRPLVWFYWIQAELVAMATDLAEFLGAA 139 Query: 145 LALHFL-GVSQFWGVLASAVIV--MLAVSTGDFRRFERF--GIVLVAGSLLLVPVILMVH 199 LA+ L G+ FWG + + V+ +L + R E VL+ G LV V+L Sbjct: 140 LAIQLLTGLPMFWGAVVTGVVTFWLLNLQKRGTRPLELAVGAFVLMIGVAYLVQVVL-AR 198 Query: 200 PPIGQIAADFFVPKM--PQDAKLGEVMLLIVAIVGTTVAPWQLFFQQSYIVDKRITP--- 254 P + + A FVP++ P A L V I+G TV P + + S + RI Sbjct: 199 PDLAAVGAG-FVPRLQGPGSAYLA------VGIIGATVMP-HVIYLHSALTQGRIQTDTT 250 Query: 255 ----RFIRYERMDLCIGIVLVVVGAVAMISFCAAVFAGK--PEFGNYTDALGTAVGLETY 308 R +R R+D+ + L + ++M++ AA F GK G+ T A T L Sbjct: 251 EEKRRLVRLNRVDVIAAMGLAGLINMSMLAVAAATFHGKNVENAGDLTTAYQT---LTPL 307 Query: 309 AGRLPAVLFALALLDACIIGAAAVSLATAYAIGDVFAVKHSLHRKPWDAKGFYSVYCGLI 368 G +VLFA+ALL + + +A ++A GDV P + ++ I Sbjct: 308 LGPAASVLFAVALLASGLSSSAVGTMA-----GDVIMQGFMGFHIPLWLRRLITMLPAFI 362 Query: 369 VLAAVLVLTPGTPLGLLTNAVQTLAGVLL----PSATVFLLLLCNDRHILG 415 V+ LG+ ++V L+ V+L P A V LLL R ++G Sbjct: 363 VIL----------LGMDPSSVLILSQVILCFGVPFALVPLLLFTARRDVMG 403