BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0016.p (289 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0221 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 256 3e-68 BS_ylbK [R] COG1752 Predicted esterase of the alpha-beta hydrola... 98 2e-20 RSc1178 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 89 8e-18 BH2587 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 88 3e-17 aq_1386 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 82 2e-15 >RSc0221 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 303 Score = 256 bits (655), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 137/283 (48%), Positives = 188/283 (66%), Gaps = 1/283 (0%) Query: 8 APKTAFVFAGGGSFGAIQVGMLQALAAHGVVADMVVGSSVGALNGAFYAGDPTVSGVARL 67 A +TAFVFAGGGS GAI+ GML+ L A GV+ DMVVG+S GA+N AF+A P + G ARL Sbjct: 4 AERTAFVFAGGGSLGAIEAGMLRELVARGVMPDMVVGASAGAINAAFFACHPHMDGAARL 63 Query: 68 AEIWRGLDRQDVFPVTWRTLVSFLWRRD-FLIPHDGVRKLIDDYIPFRNLEDARIPIHIV 126 +WRG+ R +V P +WR ++ L RR L+ G+++L++ + LE A +P+H+V Sbjct: 64 EALWRGVRRAEVMPWSWRVMLGMLGRRSTHLVDAGGLQRLLNRHFAQARLEAAPLPLHVV 123 Query: 127 TTDIISGDSVVLSEGSAAEAIVASTAIPGAFTPVHYRDHFLADGAISSNTPVRVALRHGA 186 TD+ +G VVLS GS +A++AS AIPG F PV L DG +++NTP+ A+R GA Sbjct: 124 ATDMQTGAEVVLSTGSIVQAVLASAAIPGVFPPVRVDGRLLIDGGVANNTPISTAIRLGA 183 Query: 187 KRLIVLPTGHACANQAPPVGALANALHALTLLIARQLVNELENIGPDVEYFVVPPLCPLV 246 R++VLP G ACA++ P A+ +AL+AL+LL+ARQL +LE+ + VVPPLCPL Sbjct: 184 TRVVVLPAGFACADRREPRSAIEHALNALSLLVARQLAQDLEHWAGTIPISVVPPLCPLE 243 Query: 247 GSPYDFSRTADHITRALDTTNAWLDGDGLKPGHIPHELQPHEH 289 SPYD++R + I RA T AWL DGL ++P L PH H Sbjct: 244 VSPYDYTRCGELIDRAAAATAAWLAADGLAACNVPGALYPHHH 286 >BS_ylbK [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Bsu Bacillus subtilis Length = 260 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 123/267 (46%), Gaps = 28/267 (10%) Query: 9 PKTAFVFAGGGSFGAIQVGMLQALAAHGVVADMVVGSSVGALNGAFYAGDPTVSGVARLA 68 PK GG+ G +G+L +L H + DM+ GSS+GAL G+FYA V+ + ++A Sbjct: 4 PKIGLALGSGGARGLAHLGVLSSLHKHQIEVDMIAGSSMGALVGSFYAAGHDVATMKKVA 63 Query: 69 EIWRGLDRQDVFPVTWRTLVSFLWRRDFLIPHDGVRKLIDDYIPFRNLEDARIPIHIVTT 128 + ++ R + + + D VR+L+ Y + +E+ +IP+ IV Sbjct: 64 KAFK-----------RRLYADYTVPKLGFLKGDRVRQLVHAYTFGKPIEELQIPLGIVAC 112 Query: 129 DIISGDSVVLSEGSAAEAIVASTAIPGAFTPVHYRDHFLADGAISSNTPVRVALRHGAKR 188 D+ +G+ +V +GS ++A+ AS +IPG F P L DGA+ PV V GA Sbjct: 113 DLQTGEKIVFRKGSVSDAVRASISIPGIFIPQRLDGRLLVDGAVVDRIPVSVVKDMGADI 172 Query: 189 LIVLPTGHACANQAPPVGALANALHALTLLIARQ--LVNEL---ENIGPDVEYFVVPPLC 243 +I A+ V A+H +++ L NEL + I D+ + P L Sbjct: 173 II--------ASDVSRVRKTETAVHIFDVIMQSMDILQNELVRHQTIAADI--MIRPSLE 222 Query: 244 PLVGSPYDFSRTADHITRALDTTNAWL 270 S F+ + I+ + TN + Sbjct: 223 TY--SSSSFANIEEMISAGEEATNRMI 247 >RSc1178 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 319 Score = 89.4 bits (220), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 71/263 (26%), Positives = 119/263 (45%), Gaps = 26/263 (9%) Query: 4 TDPDAP--KTAFVFAGGGSFGAIQVGMLQALAAHGVVADMVVGSSVGALNGAFYAGDPTV 61 + P P + GG + G +G+++AL A G+ D + G+S G++ A YA Sbjct: 44 SQPAVPPVRIGLALGGGAARGFAHIGVIKALEAQGIQIDFITGTSAGSVVAALYA----- 98 Query: 62 SGVARLAEIWRGLDRQDVFPVTWRTLVSFLWRRDFLIPHDGVRKLIDDYIPFRNLEDARI 121 SG++ + + L + W + F R + + + K ++ + + +E RI Sbjct: 99 SGMSGIELNRQALKMDEATIADWA--LPFGTRFGGWLKGEALEKYVNRLVKQKTIEQMRI 156 Query: 122 PIHIVTTDIISGDSVVLSEGSAAEAIVASTAIPGAFTPVHYRDHFLADGAISSNTPVRVA 181 P+ IV TD+ SG ++ G+ +A+ AS +IPG F PV H DG + + PV A Sbjct: 157 PLGIVATDLGSGRPILFRRGNTGQAVRASCSIPGVFQPVTISGHQYVDGGLVAPVPVTYA 216 Query: 182 LRHGAKRLIVL-----PTGHACANQAPPVGALANALHALTLLIARQLVNELENIGPDVEY 236 + GA +I + P+ A + QA ++ T I Q +N+ E DV Sbjct: 217 KQMGATFVIAVNISADPSAQAVSGQA--------SMLLQTTTIMGQSINKTELAQADV-- 266 Query: 237 FVVPPLCPLVGSPYDFSRTADHI 259 V+ P P V DF+ + I Sbjct: 267 -VIAPSLPFVKG-SDFTARNEAI 287 >BH2587 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Bha Bacillus halodurans Length = 275 Score = 87.8 bits (216), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 60/185 (32%), Positives = 87/185 (47%), Gaps = 25/185 (13%) Query: 9 PKTAFVFAGGGSFGAIQVGMLQALAAHGVVADMVVGSSVGALNGAFY-AGDPTVSGVARL 67 PK GG+ G +G+L+ L + D + GSS+GAL + Y AG PT + R Sbjct: 7 PKIGLALGSGGARGYAHIGVLKVLEQEKIPIDYLAGSSMGALVASLYGAGHPT-EHLIRF 65 Query: 68 AEIWRGLDRQDVFPVTWRTLVSFLWRRDFLIPHDG------VRKLIDDYIPFRNLEDARI 121 A +++ R+ + DF +P G V +LI + +E+ Sbjct: 66 ANLFK---RK--------------YYLDFTVPKMGFIAGHRVEELIRVLAKKKRVEELDP 108 Query: 122 PIHIVTTDIISGDSVVLSEGSAAEAIVASTAIPGAFTPVHYRDHFLADGAISSNTPVRVA 181 P+ IV D++ G+ V+L EG AEA+ AS AIPG F P + D L DG + PV V Sbjct: 109 PVRIVAADLLKGERVILQEGDVAEAVRASIAIPGIFVPKNINDRLLIDGGVIDRVPVSVV 168 Query: 182 LRHGA 186 GA Sbjct: 169 KEMGA 173 >aq_1386 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Aae Aquifex aeolicus Length = 259 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 46/167 (27%), Positives = 77/167 (46%), Gaps = 11/167 (6%) Query: 13 FVFAGGGSFGAIQVGMLQALAAHGVVADMVVGSSVGALNGAFYAGDPTVSGVARLAEIWR 72 V +GG + G +G+L+AL G+ + G S GA+ FYA T + +L + Sbjct: 15 LVLSGGAAKGIAHIGVLKALEELGIKVKRLSGVSAGAIVSVFYASGYTPDEMLKLLK--- 71 Query: 73 GLDRQDVFPVTWRTLVSFLWRRDFLIPHDGVRKLIDDYIPFRNLEDARIPIHIVTTDIIS 132 V W L F + L+ + + ++ + + LED IP ++ + D+ + Sbjct: 72 --------EVNWLKLFKFKTPKMGLMGWEKAAEFLEKELGVKRLEDLNIPTYLCSADLYT 123 Query: 133 GDSVVLSEGSAAEAIVASTAIPGAFTPVHYRDHFLADGAISSNTPVR 179 G ++ G ++ S +IPG F PV Y + L DG I +N PV Sbjct: 124 GKALYFGRGDLIPVLLGSCSIPGIFEPVEYENFLLVDGGIVNNLPVE 170