BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0030.p (454 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs2778 [GEPR] COG0477 Permeases of the major facilitator superf... 289 1e-77 ZshiA [GEPR] COG0477 Permeases of the major facilitator superfam... 288 2e-77 shiA [GEPR] COG0477 Permeases of the major facilitator superfami... 286 5e-77 Cgl2854 [GEPR] COG0477 Permeases of the major facilitator superf... 286 9e-77 PA3595 [GEPR] COG0477 Permeases of the major facilitator superfa... 282 1e-75 >ECs2778 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 289 bits (739), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 169/429 (39%), Positives = 242/429 (56%), Gaps = 15/429 (3%) Query: 21 PSEHQLQM---RRAVIASTVGTAIEWYDFFLYSTVTGLVFAKLFFPHSDPWVGTLEAFAI 77 P E + + RRA + S G ++WYDF LY LVF + FFP P +GTL AFA Sbjct: 10 PDERTVSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFAT 69 Query: 78 YAVGFVARPIGAAIFGHYGDRIGRKSTLIATLLLMGLATAAVALVPTYSSIGIWGAVILT 137 + VGF+ RP+G IFGH+GDR+GRK L+ T+ +MG+ATA + ++P++S+IG W ++L Sbjct: 70 FGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLV 129 Query: 138 VLRFIQGVGVGGEWGGSVLMSMEWARNDRSRGLIASWPQFGVPCGLFLANLAVLAFSQMS 197 LR IQG VGGEWGG+ L+S+E A ++ + +S Q G GL L+ V S M+ Sbjct: 130 TLRAIQGFAVGGEWGGAALLSVESAPKNK-KAFYSSGVQVGYGVGLLLSTGLVSLISMMT 188 Query: 198 GD-QFLSWGWRIPFALSIVLVGVGLYIRLGILETPVFSKLLAERQL--DRTPMLTVIRDH 254 D QFLSWGWRIPF SIVLV L++R G+ E+ F + + R P++ + H Sbjct: 189 TDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAAKKRIPVIEALLRH 248 Query: 255 PKEILLSAFARMSEQAPFYIFTAFVFSYGVGTLHVSRDFLLTAVLAASVLSFVSIPLCGH 314 P L R+ E YI TAF +Y + + R+ L L LS ++IP Sbjct: 249 PGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAW 308 Query: 315 ISDQIGRKNMYMIGAAVTGVFGFIYFAMLNTGSET-IIFLAIFLSLIPHDMQYGPQAALI 373 ++D+ GR+ +Y+ GA + + F +F L S I+F +I L+ I HDM Q + Sbjct: 309 LADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMF 368 Query: 374 AESFTGRLRYSGASLGYQLASVIAGGPAPLIA----TWLFGTFHSATAIAAYIAACAIIT 429 E F RYSGA +GYQ+ASV+ GG P IA T+ G +HS +A Y+ A +I+ Sbjct: 369 TEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHS---VAIYLLAGCLIS 425 Query: 430 LVATAMMTD 438 + +M D Sbjct: 426 AMTALLMKD 434 >ZshiA [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 288 bits (736), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 169/429 (39%), Positives = 241/429 (56%), Gaps = 15/429 (3%) Query: 21 PSEHQLQM---RRAVIASTVGTAIEWYDFFLYSTVTGLVFAKLFFPHSDPWVGTLEAFAI 77 P E + + RRA + S G ++WYDF LY LVF + FFP P +GTL AFA Sbjct: 10 PDERTVSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFAT 69 Query: 78 YAVGFVARPIGAAIFGHYGDRIGRKSTLIATLLLMGLATAAVALVPTYSSIGIWGAVILT 137 + VGF+ RP+G IFGH+GDR+GRK L+ T+ +MG+ATA + ++P++S+IG W ++L Sbjct: 70 FGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLV 129 Query: 138 VLRFIQGVGVGGEWGGSVLMSMEWARNDRSRGLIASWPQFGVPCGLFLANLAVLAFSQMS 197 LR IQG VGGEWGG+ L+S+E A ++ + +S Q G GL L+ V S M+ Sbjct: 130 TLRAIQGFAVGGEWGGAALLSVESAPKNK-KAFYSSGVQVGYGVGLLLSTGLVSLISMMT 188 Query: 198 GD-QFLSWGWRIPFALSIVLVGVGLYIRLGILETPVFSKLLAERQL--DRTPMLTVIRDH 254 D QFLSWGWRIPF SIVLV L++R G+ E+ F + + R P++ + H Sbjct: 189 TDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQYNQAAAKKRIPVIEALLRH 248 Query: 255 PKEILLSAFARMSEQAPFYIFTAFVFSYGVGTLHVSRDFLLTAVLAASVLSFVSIPLCGH 314 P L R+ E YI TAF +Y + + R+ L L LS ++IP Sbjct: 249 PGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAW 308 Query: 315 ISDQIGRKNMYMIGAAVTGVFGFIYFAMLNTGSET-IIFLAIFLSLIPHDMQYGPQAALI 373 +D+ GR+ +Y+ GA + + F +F L S I+F +I L+ I HDM Q + Sbjct: 309 XADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMF 368 Query: 374 AESFTGRLRYSGASLGYQLASVIAGGPAPLIA----TWLFGTFHSATAIAAYIAACAIIT 429 E F RYSGA +GYQ+ASV+ GG P IA T+ G +HS +A Y+ A +I+ Sbjct: 369 TEMFGASYRYSGAGVGYQVASVVXGGFTPFIAAALITYFAGNWHS---VAIYLLAGCLIS 425 Query: 430 LVATAMMTD 438 + +M D Sbjct: 426 AMTALLMKD 434 >shiA [GEPR] COG0477 Permeases of the major facilitator superfamily Eco Escherichia coli K12 Length = 438 Score = 286 bits (733), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 169/429 (39%), Positives = 241/429 (56%), Gaps = 15/429 (3%) Query: 21 PSEHQLQM---RRAVIASTVGTAIEWYDFFLYSTVTGLVFAKLFFPHSDPWVGTLEAFAI 77 P E L + RRA + S G ++WYDF LY LVF + FFP P +GTL AFA Sbjct: 10 PDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPAMGTLAAFAT 69 Query: 78 YAVGFVARPIGAAIFGHYGDRIGRKSTLIATLLLMGLATAAVALVPTYSSIGIWGAVILT 137 + VGF+ RP+G IFGH+GDR+GRK L+ T+ +MG+ATA + ++P++S+IG W ++L Sbjct: 70 FGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFSTIGWWAPILLV 129 Query: 138 VLRFIQGVGVGGEWGGSVLMSMEWARNDRSRGLIASWPQFGVPCGLFLANLAVLAFSQMS 197 LR IQG VGGEWGG+ L+S+E A ++ + +S Q G GL L+ V S M+ Sbjct: 130 TLRAIQGFAVGGEWGGAALLSVESAPKNK-KAFYSSGVQVGYGVGLLLSTGLVSLISMMT 188 Query: 198 GD-QFLSWGWRIPFALSIVLVGVGLYIRLGILETPVFSKLLAERQL--DRTPMLTVIRDH 254 D QFLSWGWRIPF SIVLV L++R G+ E+ F + + R P++ + H Sbjct: 189 TDEQFLSWGWRIPFLFSIVLVLGALWVRNGMEESAEFEQQQHYQAAAKKRIPVIEALLRH 248 Query: 255 PKEILLSAFARMSEQAPFYIFTAFVFSYGVGTLHVSRDFLLTAVLAASVLSFVSIPLCGH 314 P L R+ E YI TAF +Y + + R+ L L LS ++IP Sbjct: 249 PGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAW 308 Query: 315 ISDQIGRKNMYMIGAAVTGVFGFIYFAMLNTGSET-IIFLAIFLSLIPHDMQYGPQAALI 373 ++D+ GR+ +Y+ G + + F +F L S I+F +I L+ I HDM Q + Sbjct: 309 LADRFGRRRVYITGTLIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMF 368 Query: 374 AESFTGRLRYSGASLGYQLASVIAGGPAPLIA----TWLFGTFHSATAIAAYIAACAIIT 429 E F RYSGA +GYQ+ASV+ GG P IA T+ G +HS +A Y+ A +I+ Sbjct: 369 TEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHS---VAIYLLAGCLIS 425 Query: 430 LVATAMMTD 438 + +M D Sbjct: 426 AMTALLMKD 434 >Cgl2854 [GEPR] COG0477 Permeases of the major facilitator superfamily Cgl Corynebacterium glutamicum Length = 433 Score = 286 bits (731), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 172/429 (40%), Positives = 269/429 (62%), Gaps = 12/429 (2%) Query: 10 LTPGLDANRLTPSEHQLQMRRAVIASTVGTAIEWYDFFLYSTVTGLVFAKLFFPHSDPWV 69 +T +D N +P + QL +R ++ S G+ IEW+DF +Y TV LVF K++FP + ++ Sbjct: 1 MTTAVDQN--SPPKQQLN-KRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSGNEFL 57 Query: 70 GTLEAFAIYAVGFVARPIGAAIFGHYGDRIGRKSTLIATLLLMGLATAAVALVPTYSSIG 129 T+ A+A +++ F RPIG IF H GDRIGRK TL TL+LMG T A+ L+P Y++IG Sbjct: 58 STILAYASFSLTFFFRPIGGVIFAHIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNAIG 117 Query: 130 IWGAVILTVLRFIQGVGVGGEWGGSVLMSMEWARNDRSRGLIASWPQFGVPCGLFLANLA 189 IW ++L LR +QG+G+GGEWGG++L++ E+A + RGL + PQ G+ G+ LA Sbjct: 118 IWAPILLMFLRILQGIGIGGEWGGALLLAYEYAPK-KQRGLYGAVPQMGISLGMLLAAGV 176 Query: 190 VLAFSQMSGDQFLSWGWRIPFALSIVLVGVGLYIRLGILETPVFSKLLAERQLDRTPMLT 249 + + M DQFL+WGWRIPF SI+LV +GL+IR G+ ETP F ++ Q + P+ Sbjct: 177 ISLLTLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKE 236 Query: 250 VIRDHPKEILLSAFARMSEQAPFYIFTAFVFSYGVGTLHVSRDFLLTAVLAASVLSFVSI 309 V+ + +L+S A+ +E PFYIF ++ +Y L++ + +L AV A++++ + + Sbjct: 237 VLTKYWPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWM 296 Query: 310 PLCGHISDQIGRKNMYMIGAAVTGVFGFIYFAMLNTGSETIIFLAIFLSLIPHDMQYGPQ 369 PL G SD++ R +Y I A+ T V Y+ +LNTG +F+ ++I + +G Sbjct: 297 PLFGSFSDRVNRAVLYRICASATIVLIVPYYLVLNTGEIWALFIT---TVIGFGILWGSV 353 Query: 370 AAL----IAESFTGRLRYSGASLGYQLASVIAGGPAPLIATWLFGTFHSA-TAIAAYIAA 424 A+ IAE+F +RY+GA+LGYQ+ + + GG AP+IA WLF IA Y+AA Sbjct: 354 NAILGTVIAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEISGGQWWPIAVYVAA 413 Query: 425 CAIITLVAT 433 C +++++A+ Sbjct: 414 CCLLSVIAS 422 >PA3595 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 469 Score = 282 bits (722), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 167/439 (38%), Positives = 250/439 (56%), Gaps = 16/439 (3%) Query: 20 TPSEHQL--QMRRAVIASTVGTAIEWYDFFLYSTVTGLVFAKLFFPHS-DPWVGTLEAFA 76 +P + L + RRA IAS +GT IEWYDF++Y LVF K+FFP DP + TL +F Sbjct: 6 SPEDADLHKKARRAGIASFIGTTIEWYDFYIYGLAAALVFGKVFFPADMDPGLATLLSFV 65 Query: 77 IYAVGFVARPIGAAIFGHYGDRIGRKSTLIATLLLMGLATAAVALVPTYSSIGIWGAVIL 136 GF+ARP+G IFGH GDRIGRK+TL+ TL++MG+AT + L+P Y+ IGIW + L Sbjct: 66 TLWSGFIARPLGGIIFGHLGDRIGRKTTLVITLIMMGIATTCIGLLPVYTQIGIWAPISL 125 Query: 137 TVLRFIQGVGVGGEWGGSVLMSMEWARNDRSRGLIASWPQFGVPCGLFLANLAVLAFSQM 196 LR +QG+ VGGEWGG++L++ E A + L A++ Q G P G LA LA A S + Sbjct: 126 VALRIVQGIAVGGEWGGAILIASESAPKGKGI-LYAAFAQQGSPTGNLLATLAFFALSAL 184 Query: 197 SGDQFLSWGWRIPFALSIVLVGVGLYIRLGILETPVFSKLLAERQLDRTPMLTVIRDHPK 256 F+ WGWR PF LS VLV VG+ IRL + E+ ++LA+++ + P+ V+ H K Sbjct: 185 PASDFILWGWRAPFLLSAVLVIVGMVIRLKLEESEDMKRVLAQKRTVKLPLAEVLGKHWK 244 Query: 257 EILLSAFARMSEQAPFYIFTAFVFSYGVGTLHVSRDFLLTAVLAASVLSFVSIPLCGHIS 316 +LL A ++ T F S+ TL S+ L+ ++ A V+ F+S PL ++ Sbjct: 245 IVLLGAGVLPIIHVTYFKST-FALSWATKTLGYSQGTFLSIIVVALVVQFLSQPLGALLT 303 Query: 317 DQIGRKNMYMIGAAVTGVFGFIYFAMLNTGSETIIF----LAIFLSLIPHDMQYGPQAAL 372 +I + A + + FI + ET ++ + + L+ +PH M YG + Sbjct: 304 SRIDMRK----AACLMVLPEFILMPAMFFAVETKVYWIAAVGMCLATVPHAMFYGAVGGI 359 Query: 373 IAESFTGRLRYSGASLGYQLASVIAGGPAPLIATWLFGTFHSATAIAAYIAACAIITLVA 432 +A +F R+RY+G SL YQL S++ GG P++A W+ + S +A A A+++L Sbjct: 360 LARAFPARVRYTGLSLAYQLCSLLVGGGTPVLAQWILNSTGSIVGVALASACYALVSLAC 419 Query: 433 TAMMTDYTG---RDINAPE 448 T + TG D++ E Sbjct: 420 TLALLRITGFHASDLSTAE 438