BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0010.p (227 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2491 [IQR] COG1028 Dehydrogenases with different specificities... 137 2e-32 CAC3574 [IQR] COG1028 Dehydrogenases with different specificitie... 133 3e-31 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 130 2e-30 L0185 [IQR] COG1028 Dehydrogenases with different specificities ... 130 2e-30 CAC2607 [IQR] COG1028 Dehydrogenases with different specificitie... 127 3e-29 >BH2491 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 137 bits (344), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 83/193 (43%), Positives = 115/193 (59%), Gaps = 3/193 (1%) Query: 7 LLAGKRALVTGAAHGNGRAIALGLAAHGADVVVTDI-DRAGAEQTAADIRQHGRAAWPFE 65 +L GK A+VTGA+ G GRA A+ LA HGA+VVV ++ AE+ A+I++ G A + Sbjct: 1 MLQGKTAIVTGASRGIGRATAMELARHGANVVVNYAGNKEKAEKVVAEIKELGVEAIAIQ 60 Query: 66 LDVTDAEACSALAQRAAREVGPIAILVNNAGIIIREGIDSPRARENWRKVLDVNLTGTFN 125 DV D+E+ A+ + G + ILVNNAGI R+ + E+W V+D NL G F+ Sbjct: 61 ADVADSESVQAMVKETIDTFGAVDILVNNAGIT-RDNLFMRMKEEDWDAVIDTNLKGVFH 119 Query: 126 VTHAFL-PALRETRGTIINLGSIASFLGVADTLGYAPSKGGVKLLTQALARELAADGIRV 184 + A P +++ G IIN+ S+ +G A Y +K GV LT+ LARELA I V Sbjct: 120 CSKAVTRPMMKQRFGRIINVSSVVGAIGNAGQANYVAAKAGVIGLTKTLARELANRNITV 179 Query: 185 NAIAPGVIETAMT 197 NA+APG IET MT Sbjct: 180 NAVAPGFIETDMT 192 >CAC3574 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 249 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 78/198 (39%), Positives = 121/198 (61%), Gaps = 3/198 (1%) Query: 4 MATLLAGKRALVTGAAHGNGRAIALGLAAHGADVVVT-DIDRAGAEQTAADIRQHGRAAW 62 M LL+GK A+VTGA G GRAIAL LAA GA++VV A ++ +I + G A Sbjct: 1 MEKLLSGKVAVVTGAGRGLGRAIALKLAAEGANLVVNYRSSEAETQKLIKEIEELGSKAV 60 Query: 63 PFELDVTDAEACSALAQRAAREVGPIAILVNNAGIIIREGIDSPRARENWRKVLDVNLTG 122 + D++ + + ++A E G + ILVNNAGI ++ + E++ V++VNL G Sbjct: 61 AVKADISKYDEAETIIKKALDEYGTVDILVNNAGIT-KDNLLLRMKEEDFDSVINVNLKG 119 Query: 123 TFN-VTHAFLPALRETRGTIINLGSIASFLGVADTLGYAPSKGGVKLLTQALARELAADG 181 FN + H L++ G IIN+ S+ +G A + YA +K G+ +T+++A+ELA+ G Sbjct: 120 AFNCIKHTSRVMLKKKSGKIINISSVIGLIGNAGQVNYAAAKAGIIGMTKSVAKELASRG 179 Query: 182 IRVNAIAPGVIETAMTEA 199 I VNA+APG+I++ MT+A Sbjct: 180 ITVNAVAPGIIKSDMTDA 197 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 246 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 76/192 (39%), Positives = 107/192 (55%), Gaps = 2/192 (1%) Query: 8 LAGKRALVTGAAHGNGRAIALGLAAHGADVVVTDIDRAGAEQTAADIRQHGRAAWPFELD 67 L GK L+TGAA G G+A L A GA V+ DI + + + P+ L+ Sbjct: 3 LEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYVLN 62 Query: 68 VTDAEACSALAQRAAREVGPIAILVNNAGIIIREGIDSPRARENWRKVLDVNLTGTFNVT 127 VTD + + ++ ++ G I +LVNNAGI R+ + E+W V++VNL G FNVT Sbjct: 63 VTDRDQIKEVVEKVVQKYGRIDVLVNNAGIT-RDALLVRMKEEDWDAVINVNLKGVFNVT 121 Query: 128 HAFLPALRETR-GTIINLGSIASFLGVADTLGYAPSKGGVKLLTQALARELAADGIRVNA 186 +P + + R G+I+N+ S+ G YA SK GV +T+ A+ELA IRVNA Sbjct: 122 QMVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNA 181 Query: 187 IAPGVIETAMTE 198 +APG IET MTE Sbjct: 182 VAPGFIETPMTE 193 >L0185 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Lla Lactococcus lactis Length = 243 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 76/192 (39%), Positives = 112/192 (58%), Gaps = 3/192 (1%) Query: 8 LAGKRALVTGAAHGNGRAIALGLAAHGADVVVTDIDRAGAEQTAADIRQHGRAAWPFELD 67 + K VTG+ G G+AIAL A G+++++ A +E+ A+ +G A D Sbjct: 3 IKNKNVFVTGSTRGIGKAIALQFAKAGSNLIING-RSAISEELLAEFTAYGVKAVGISGD 61 Query: 68 VTDAEACSALAQRAAREVGPIAILVNNAGIIIREGIDSPRARENWRKVLDVNLTGTFNVT 127 ++ +E + A +G + ILVNNAGI R+G+ + E++ VL +NLTG FN+T Sbjct: 62 ISKSEDAKQMVAEAIETLGSVDILVNNAGIT-RDGLSLKMSEEDFESVLKINLTGAFNMT 120 Query: 128 HAFL-PALRETRGTIINLGSIASFLGVADTLGYAPSKGGVKLLTQALARELAADGIRVNA 186 A L P R G IIN+ S+ +G A YA SK G+ LT+++ARE+AA +RVNA Sbjct: 121 QAVLKPMTRARSGAIINISSVVGLMGNAGQANYAASKAGLIGLTKSIAREVAARNVRVNA 180 Query: 187 IAPGVIETAMTE 198 +APG IE+ MTE Sbjct: 181 VAPGFIESDMTE 192 >CAC2607 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 267 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 79/195 (40%), Positives = 111/195 (56%), Gaps = 2/195 (1%) Query: 8 LAGKRALVTGAAHGNGRAIALGLAAHGADVVVTDIDRAGAEQTAADIRQHGRAAWPFELD 67 L GK ALVTGA++G G AIA A GA +V DI++ ++ G A + D Sbjct: 9 LEGKIALVTGASYGIGFAIARSYANAGATIVFNDINQDLVDKGLKAYEAEGIKAHGYVCD 68 Query: 68 VTDAEACSALAQRAAREVGPIAILVNNAGIIIREGIDSPRARENWRKVLDVNLTGTFNVT 127 VTD + + ++ +EVG I ILVNNAGII R + +A E++RKV+DV+L F V+ Sbjct: 69 VTDEDKVNGFVKKVEKEVGLIDILVNNAGIIKRIPMLEMKA-EDFRKVIDVDLNAPFIVS 127 Query: 128 HAFLPAL-RETRGTIINLGSIASFLGVADTLGYAPSKGGVKLLTQALARELAADGIRVNA 186 A +P + ++ G IIN+ S+ S LG YA +KGG+K+LT+ +A E I+ N Sbjct: 128 KAVIPGMIKKGHGKIINICSMMSELGRETVSAYAAAKGGLKMLTRNIASEYGEHNIQCNG 187 Query: 187 IAPGVIETAMTEATR 201 I PG I T T R Sbjct: 188 IGPGYIATPQTAPLR 202