BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0058.p (620 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming... 282 2e-75 HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 261 2e-69 PAE2464_1 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-formi... 249 2e-65 VC2484 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 238 5e-62 PM0925 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 237 5e-62 >APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Ape Aeropyrum pernix Length = 651 Score = 282 bits (722), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 168/490 (34%), Positives = 259/490 (52%), Gaps = 14/490 (2%) Query: 22 QTRGDRPAIREKKFGIWQPTSWREWLQISKDIAFGLQATGFRPGDVASIIANAVPEWVYA 81 +T+ R A R K++GIW+ +WR++ A L+ G PGD + I P WV Sbjct: 32 RTQPGRVAFRWKRYGIWRRYTWRDYYLRVAYAALALENLGLGPGDTGAFITFNRPSWVIY 91 Query: 82 DMGILCAGGVSSGIYXTKKKNDSAAQVEYLINDSRTRVIFVEDEEQLDKVLTCRARCPTL 141 ++G+ GG + GIY ++ + +V YL++ + + VE +EQLD+VL + Sbjct: 92 EVGVQLLGGRAMGIY----RDSLSDEVGYLLDSGDAKFVLVEGQEQLDRVLDSGV---DV 144 Query: 142 EKIVVFDMEGLS---GFSDPMVLSFAEFTALGRN-HAQGNDAVWDEMTGSRTASDLAILV 197 +KI+V + +GL G +++F + LGR ++ G D ++ G + L Sbjct: 145 DKIIVDEAKGLHQYRGMVGSKLVTFGDVMRLGRKLYSDGGDQQVKKLMGELEPDMVCGLF 204 Query: 198 YTSGTTGPPKGAMHSNRSVTHQMRHANDLYPSTDSEERLVFLPLCHVAERVGGYYISLAL 257 TSGTTG PK AM S +++ N + P + E + FLP + E++ + Sbjct: 205 TTSGTTGLPKLAMISFKNMLAMAHQLNTVDPVREDWEYVSFLPTAWIGEQMMSIPYHMIG 264 Query: 258 GSVMNFAESPETVPDNLREVQPTAFLAVPRIWEKFYSGITIALKDATPFQNWMYARALAI 317 G +NF E+P T+ + RE+ P + PR+WE+ I + DA P + + A I Sbjct: 265 GFKVNFPETPHTLWRDFREIAPHFMFSPPRVWERIAKDIMARVDDADPIKRAAFRLAYKI 324 Query: 318 GNRMTECRL-QGEA-PPLLLRIANRAAYWLVFRNIRRMLGLDRCRIALTGAAPISPDLIR 375 G RL +G + PP L R AYWL R + GL R A TG A I D + Sbjct: 325 GYEAARRRLVKGRSRPPTLWRALYYLAYWLALRAVLDKAGLKRIVRAYTGGAMIGEDYVI 384 Query: 376 WYLALGIDMREVYGQTENCGVATMMPADRIKLGSVGKAAPWGEVMICPKGEILIKGDFLF 435 +Y +LG++++++YGQTE G+A + P D ++ +VGK P EV I GEIL++ + Sbjct: 385 FYHSLGVNLKQIYGQTEVAGIAVVHPDDDVRPDTVGKPLPLTEVKIAEDGEILMRSPAMM 444 Query: 436 MGYLNQPEKTAETIDAKGWLHTGDVGTIDNEGYVKITDRMKDIIITSGGKNITPSEIENQ 495 GY E TA+TI GWL TGDVG + +G++K+ DR K+III S G + P I+N+ Sbjct: 445 KGYYKNEEATAKTI-VDGWLRTGDVGVLTEDGHLKVLDRAKEIIILSDGTKVAPQVIQNK 503 Query: 496 LKFSPYVSDA 505 LKFSPY+ +A Sbjct: 504 LKFSPYIGEA 513 >HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Hin Haemophilus influenzae Length = 607 Score = 261 bits (668), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 151/478 (31%), Positives = 246/478 (51%), Gaps = 16/478 (3%) Query: 22 QTRGDRPAIREKKFGIWQPTSWREWLQISKDIAFGLQATGFRPGDVASIIANAVPEWVYA 81 +TR + A+R K+ G+W+ SW+ + + ++ L A D +I A+ + W Sbjct: 24 KTRTNMTALRYKEHGLWRDISWKNFQEQLNQLSRALLAHNIDVQDKIAIFAHNMERWTIV 83 Query: 82 DMGILCAGGVSSGIYXTKKKNDSAAQVEYLINDSRTRVIFVEDEEQLDKVLTCRARCPTL 141 D+ L ++ IY T ++A Q E+++N + +++FV D+EQ D+ L CP L Sbjct: 84 DIATLQIRAITVPIYAT----NTAQQAEFILNHADVKILFVGDQEQYDQTLEIAHHCPKL 139 Query: 142 EKIVVFDMEGLSGFSDPMVLSFAEFTALGRNHAQGNDAVWDEMT---GSRTASDLAILVY 198 +KIV + DP+ ++ F G N Q DE+T + SDL ++Y Sbjct: 140 QKIVAMK-STIQLQQDPLSCTWESFIKTGSNAQQ------DELTQRLNQKQLSDLFTIIY 192 Query: 199 TSGTTGPPKGAMHSNRSVTHQMRHANDLYPSTDSEERLVFLPLCHVAERVGGYYISLALG 258 TSGTTG PKG M ++ HQ+ + TD + L FLP H+ ER YI L G Sbjct: 193 TSGTTGEPKGVMLDYANLAHQLETHDLSLNVTDQDISLSFLPFSHIFERAWAAYI-LHRG 251 Query: 259 SVMNFAESPETVPDNLREVQPTAFLAVPRIWEKFYSGITIALKDATPFQNWMYARALAIG 318 +++ + E V L E++PT AVPR +EK Y+ + ++ A + M+ A+++G Sbjct: 252 AILCYLEDTNQVRSALTEIRPTLMCAVPRFYEKIYAAVLDKVQKAPKLRQIMFHWAISVG 311 Query: 319 NRMTECRLQGEAPPLLLRIANRAAYWLVFRNIRRMLGLDRCRIALTGAAPISPDLIRWYL 378 + + R +A P LL+ A LV +R++LG R ++ G A + P + ++ Sbjct: 312 QKYFDLRANNKAIPFLLKKQFALADKLVLSKLRQLLG-GRIKMMPCGGAKLEPAIGLFFH 370 Query: 379 ALGIDMREVYGQTENCGVATMMPADRIKLGSVGKAAPWGEVMICPKGEILIKGDFLFMGY 438 A+GI+++ YG TE + + S+G P EV I EIL++G + GY Sbjct: 371 AIGINIKLGYGMTETTATVSCWHDFQFNPNSIGTLMPKAEVKIGENNEILVRGGMVMKGY 430 Query: 439 LNQPEKTAETIDAKGWLHTGDVGTIDNEGYVKITDRMKDIIITSGGKNITPSEIENQL 496 +PE+TA+ G+L TGD G D +G + ITDR+K+++ TS GK I P IE+++ Sbjct: 431 YKKPEETAQAFTEDGFLKTGDAGEFDEQGNLFITDRIKELMKTSNGKYIAPQYIESKI 488 >PAE2464_1 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Pya Pyrobaculum aerophilum Length = 448 Score = 249 bits (635), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 134/424 (31%), Positives = 227/424 (53%), Gaps = 7/424 (1%) Query: 29 AIREKKFGIWQPTSWREWLQISKDIAFGLQATGFRPGDVASIIANAVPEWVYADMGILCA 88 A REK +GIW+PT+W E+ + A+GL A GFR GD +++ PE + A+ + Sbjct: 29 AFREKTWGIWRPTTWHEYYERVSKFAYGLLALGFRRGDHLAVVGYNRPEILIAEQSAMAL 88 Query: 89 GGVSSGIYXTKKKNDSAAQVEYLINDSRTRVIFVEDEEQLDKVLTCRARCPTLEKIVVFD 148 GG+S G+Y + A ++Y + +V+ ED+EQ+DK+L + P LE I+ +D Sbjct: 89 GGISVGVYPDTLPEERAYWLDY----TDVKVVVAEDQEQVDKILVVKKDLPKLEYIIYWD 144 Query: 149 MEGLSGFSD---PMVLSFAEFTALGRNHAQGNDAVWDEMTGSRTASDLAILVYTSGTTGP 205 + + D P +LS+ E LG + + + +E+ D+ +++ TSGTTG Sbjct: 145 ERMMWQYKDIKEPGLLSWKEVEKLGEEVRKSDSKLLEELIAKTHPDDVCLILSTSGTTGR 204 Query: 206 PKGAMHSNRSVTHQMRHANDLYPSTDSEERLVFLPLCHVAERVGGYYISLALGSVMNFAE 265 PKG M + S+ ++ ++ P ++ E + +LP + E++ + + +G +NF E Sbjct: 205 PKGVMLTYSSMMSMAKNLWEVDPIQENFEYVSYLPFGWIGEQMMSLAMHMLVGFKINFVE 264 Query: 266 SPETVPDNLREVQPTAFLAVPRIWEKFYSGITIALKDATPFQNWMYARALAIGNRMTECR 325 PET + RE+ P R++E YS I L+D F ++ AL IG R E Sbjct: 265 EPETFWRDFREIAPHFMFGPARVYEMIYSKIMEHLEDTWDFDKKIFEIALNIGYRKVEAE 324 Query: 326 LQGEAPPLLLRIANRAAYWLVFRNIRRMLGLDRCRIALTGAAPISPDLIRWYLALGIDMR 385 L G+ P L + + L++R+I GL R + A G + + PD +++Y ALG++++ Sbjct: 325 LTGKRIPFWLTLLWHISRMLIYRSILDKTGLKRIKYAYVGGSFLGPDYLKFYRALGVNLK 384 Query: 386 EVYGQTENCGVATMMPADRIKLGSVGKAAPWGEVMICPKGEILIKGDFLFMGYLNQPEKT 445 ++G TE G+AT+ P + ++L +VGK E+ I GEIL++ + GY +PE T Sbjct: 385 RIWGMTEVSGIATVHPDNEVRLDTVGKPIANTEIKIAEDGEILVRTPAVMAGYYKRPEAT 444 Query: 446 AETI 449 AE + Sbjct: 445 AEAL 448 >VC2484 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Vch Vibrio cholerae Length = 601 Score = 238 bits (606), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 149/469 (31%), Positives = 236/469 (50%), Gaps = 10/469 (2%) Query: 25 GDRPAIREKKFGIWQPTSWREWLQISKDIAFGLQATGFRPGDVASIIANAVPEWVYADMG 84 GD A++ K+ G WQ +W ++ + +++ L A G D +I +N +P W AD Sbjct: 20 GDNAALKHKQNGQWQAINWHQFGEQLDELSMALLAQGIGVQDKIAIFSNNMPRWTIADFA 79 Query: 85 ILCAGGVSSGIYXTKKKNDSAAQVEYLINDSRTRVIFVEDEEQLDKVLTCRARCPTLEKI 144 L V+ IY T ++ Q Y++ ++ +V+FV ++ Q D L+ +CP L I Sbjct: 80 ALQIRAVTVPIYPT----NTPEQAAYILQNADVKVVFVGEQAQFDAALSQFEQCPELRLI 135 Query: 145 VVFDMEGLSGFSDPMVLSFAEFTALGRNHAQGNDAVWDEMTGSRTASDLAILVYTSGTTG 204 V + + + + EF A ++H Q + A D + DL L+YTSGTTG Sbjct: 136 VAMN-ANIDLKQASCAMHWDEFVA--QSHHQ-DRAPLDALIEQANYDDLFTLIYTSGTTG 191 Query: 205 PPKGAMHSNRSVTHQMRHANDLYPSTDSEERLVFLPLCHVAERVGGYYISLALGSVMNFA 264 PKG M R++ Q+ + T + L FLPL HV ER Y+ L G+ + Sbjct: 192 TPKGVMLDYRNIGAQLEGHDQRLNLTQEDVSLCFLPLSHVFERAWTAYV-LYKGATNCYL 250 Query: 265 ESPETVPDNLREVQPTAFLAVPRIWEKFYSGITIALKDATPFQNWMYARALAIGNRMTEC 324 + V + L EV+PT AVPR +EK +S I + A + ++ A+ +G +M C Sbjct: 251 QDVAHVREALAEVRPTVMCAVPRFYEKIFSAIHEKVVKAPLVRKVLFTWAVNMGAKMAVC 310 Query: 325 RLQGEAPPLLLRIANRAAYWLVFRNIRRMLGLDRCRIALTGAAPISPDLIRWYLALGIDM 384 R Q P +L+ +++ A LV +R +LG R G A + + R++ A+GI++ Sbjct: 311 RQQQRQPSWMLKQSHQFADKLVLSKLRALLG-GRINFMPCGGAKLDETIGRFFHAIGINV 369 Query: 385 REVYGQTENCGVATMMPADRIKLGSVGKAAPWGEVMICPKGEILIKGDFLFMGYLNQPEK 444 + YG TE + S+G + P +V I EIL++G + GY ++ Sbjct: 370 KLGYGMTETTATVSCWDDHCFNPDSIGLSMPGAQVKIGENNEILVRGPMVMRGYYKLDKE 429 Query: 445 TAETIDAKGWLHTGDVGTIDNEGYVKITDRMKDIIITSGGKNITPSEIE 493 T E+ D +G+L TGD G ID G + ITDR+K+++ TSGGK I P IE Sbjct: 430 TTESFDEQGFLKTGDAGHIDENGNLFITDRIKELMKTSGGKYIAPQVIE 478 >PM0925 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Pmu Pasteurella multocida Length = 606 Score = 237 bits (605), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 143/462 (30%), Positives = 237/462 (51%), Gaps = 13/462 (2%) Query: 38 WQPTSWREWLQISKDIAFGLQATGFRPGDVASIIANAVPEWVYADMGILCAGGVSSGIYX 97 W+ SW ++ Q ++ L A D +I A+ +P W AD+G + V IY Sbjct: 33 WENISWHQFQQDLDTFSYALLANHIGVQDKIAIFAHNMPRWTIADIGTMQVRAVVVPIYA 92 Query: 98 TKKKNDSAAQVEYLINDSRTRVIFVEDEEQLDKVLTCRARCPTLEKIVVFDMEGLSGFSD 157 T +A QVEY++N++ +++FV D+E+ + L CP ++KIV + L + Sbjct: 93 T----SAAKQVEYILNNADVKILFVGDQEEYNCTLEIIDACPQIQKIVAMK-DNLDLKNH 147 Query: 158 PMVLSFAEFTALGRNHAQGNDAVWDEMTGSRTASDLAILVYTSGTTGPPKGAMHSNRSVT 217 P + F G Q E + +DL L+YTSGTTG PKG M ++ Sbjct: 148 PKACDWQTFLLEGSPLQQ---TALQERLEQKQLTDLFTLIYTSGTTGEPKGVMLDYANLA 204 Query: 218 HQMRHANDLYPSTDSEE---RLVFLPLCHVAERVGGYYISLALGSVMNFAESPETVPDNL 274 HQ++ ++ + + + + L FLPL H+ ER Y+ L G+V + E V + L Sbjct: 205 HQLKAHDEAFTTLNVSQYDSSLSFLPLSHIFERAWVAYV-LHRGAVNCYLEDTNRVREAL 263 Query: 275 REVQPTAFLAVPRIWEKFYSGITIALKDATPFQNWMYARALAIGNRMTECRLQGEAPPLL 334 E++PT AVPR +EK Y+ + ++ A F+ ++ A+A+G + + Q + P + Sbjct: 264 SEIRPTLMCAVPRFYEKIYTAVWDKVQKAPLFRRMIFNWAIAVGQKRFQFISQKKPIPFV 323 Query: 335 LRIANRAAYWLVFRNIRRMLGLDRCRIALTGAAPISPDLIRWYLALGIDMREVYGQTENC 394 LR A LV +R++LG R R+ G A + P++ ++ ++GI+++ YG TE Sbjct: 324 LRQQYALADKLVLSKLRQLLG-GRIRMMPCGGAKLEPNIGLFFHSIGINVKLGYGMTETT 382 Query: 395 GVATMMPADRIKLGSVGKAAPWGEVMICPKGEILIKGDFLFMGYLNQPEKTAETIDAKGW 454 + + S+G P EV I EIL++G + GY +P++TA++ G+ Sbjct: 383 ATVSCWEEGHFEPNSIGTLMPGAEVKIGENNEILVRGGMVMRGYYKKPQETADSFTEDGF 442 Query: 455 LHTGDVGTIDNEGYVKITDRMKDIIITSGGKNITPSEIENQL 496 L TGD G D +G + ITDR+K+++ TS GK I P IE ++ Sbjct: 443 LKTGDAGEFDPQGNLYITDRIKELMKTSNGKYIAPQYIETKV 484