BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0038.p (332 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the T... 395 e-110 mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the T... 202 7e-52 SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the T... 175 2e-43 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 67 7e-11 >mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 357 Score = 395 bits (1015), Expect = e-110, Method: Compositional matrix adjust. Identities = 185/328 (56%), Positives = 239/328 (72%), Gaps = 2/328 (0%) Query: 1 MNDYWRDQVTTRGM--TDLISQSYPTNSPISSRPDWRPAQGKPGSSLADMQRQALDRFGT 58 ++DYWR+ V RG+ DL SYP + P+S RPDWRP QG PGS ++ Q LD F + Sbjct: 30 LDDYWREHVIRRGLDREDLELSSYPPSLPLSCRPDWRPQQGLPGSQFEQLRDQVLDPFQS 89 Query: 59 AYGICNPLYGVQMVFSEDMADAFCRALNDWLVKEWLDKDDRLRGSIVIPVQSIEKAVAEI 118 + ICN L+G Q++FSED++ A CRA+N+W+ EWLD+D RLR SIV+PV S E AVAEI Sbjct: 90 RFAICNVLHGSQIMFSEDLSAALCRAINNWISAEWLDRDPRLRASIVVPVHSPELAVAEI 149 Query: 119 ERCAQDKRFVQVLMLVMGDMPLGKRHYWPIYAAAERLGLPVGIHAGSAYHNPPTSVGWGS 178 E A D RFVQVLML MG++PLG+R WPIYAAAER LP+GIHAGS+ NPP+ +GW S Sbjct: 150 EHRAADLRFVQVLMLAMGEVPLGRRQNWPIYAAAERHDLPIGIHAGSSLRNPPSPLGWSS 209 Query: 179 YHIEDYVAQAQAFQTQLTSLIVEGVFARHPNLKMVMLESGFTWLPPFLWRLHKFWRGVRM 238 ++I+DYV+QA F L SL+VEGVF + P LK+V+LESG TWLP LWR +K WRGVR Sbjct: 210 HYIDDYVSQAPGFSGALNSLVVEGVFNKFPRLKVVLLESGVTWLPAHLWRANKQWRGVRS 269 Query: 239 ETPWVDRAPLEIVRSNIRFSLQPVDAPPDGDTLNRLFDHMQSDELILFSSDYPHWQFDGD 298 ETPW+ R P EI+R ++R ++QP DAPP + + D + SDE++LFS+DYPHW FDG Sbjct: 270 ETPWLTRPPSEIIREHVRLTVQPFDAPPTDGQVQTIIDQIGSDEMLLFSTDYPHWHFDGT 329 Query: 299 EVLPQGLSQDLVRKIMIDNPHATYGRLK 326 + +PQGLS + + K++ DN ATY RL Sbjct: 330 DAVPQGLSHESLSKMLADNALATYPRLN 357 >mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 371 Score = 202 bits (515), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 127/327 (38%), Positives = 168/327 (51%), Gaps = 8/327 (2%) Query: 4 YWRDQVTTRGMTDLISQSYPTNSPISS-----RPDWRPAQGK-PGSSLADMQRQALDRFG 57 YWR+ T G + N P S R D P+ G PG+ L MQ+Q LD F Sbjct: 42 YWREHRETIGSRWRQGLAKTMNYPRMSKGGGVRQDAWPSNGCVPGADLPMMQKQLLDLFN 101 Query: 58 TAYGICNPLYGVQMVFSEDMADAFCRALNDWLVKEWLDKDDRLRGSIVIPVQSIEKAVAE 117 YGI + + + A A N+W + W D + RL+G++ I V+ + A+AE Sbjct: 102 VEYGIMVQPMELSSERNVEFGAALATAANEWQLAHWCDPEPRLKGAVQINVEDEKAAIAE 161 Query: 118 IERCAQDKRFVQVLMLVMGDMPLGKRHYWPIYAAAERLGLPVGIHAGSAYHNPPTSVGWG 177 IER A D+RFVQV G P+G+R YWPI A G P+ IH G +P T+ GW Sbjct: 162 IERRAGDRRFVQVQFPQRGVEPIGRRRYWPIMEACAANGFPIAIHIGGIGGHPSTAGGWP 221 Query: 178 SYHIEDYVAQAQAFQTQLTSLIVEGVFARHPNLKMVMLESGFTWLPPFLWRLHKFWRGVR 237 SY+ E++ A QA Q + SL+ EGV + PNLK+++LESGF WLP WRL W+ +R Sbjct: 222 SYYHEEHPAYVQAMQNTVISLVCEGVLSHIPNLKVILLESGFGWLPSLCWRLDTAWKRLR 281 Query: 238 METPWVDRAPLEIVRSNIRFSLQPVDAPPDGDTLNRLFDHMQSDELILFSSDYPHW-QFD 296 E P + P E VR I + QPVD P L + + D L +FS+DYPHW Q D Sbjct: 282 AEVPLLREKPSEYVRERIWLTTQPVDEPERPQDLLSTIEWIGEDRL-MFSTDYPHWDQDD 340 Query: 297 GDEVLPQGLSQDLVRKIMIDNPHATYG 323 L + RKI DN YG Sbjct: 341 PRYAFKAALPEAWKRKIFHDNARTLYG 367 >SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 366 Score = 175 bits (443), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 96/272 (35%), Positives = 143/272 (52%), Gaps = 8/272 (2%) Query: 30 SRPDWRPAQGKPGSSLADMQRQALDRFGTAYGICN---PLYGVQMVFSEDMADAFCRALN 86 SR D P + + A M+ + LD++ I P+ + + A+A CRA N Sbjct: 62 SRHDVNPVEED--DNYAIMKEKHLDKYNIDVAILTGDEPIEASTLA-NAHYANALCRAYN 118 Query: 87 DWLVKEWLDKDDRLRGSIVIPVQSIEKAVAEIERCAQDKRFVQVLMLVMGDMPLGKRHYW 146 D+++ WL KD R GSI++ Q + A EI R R VQVL+ P G Y Sbjct: 119 DYMIDYWLPKDSRFWGSIIVAPQDPKLAAEEIRRLGSHPRIVQVLVSHGAQRPYGDPFYH 178 Query: 147 PIYAAAERLGLPVGIHAGS--AYHNPPTSVGWGSYHIEDYVAQAQAFQTQLTSLIVEGVF 204 PIY A +GLP +H G ++ P G ++ E + Q+ T + SLI +GVF Sbjct: 179 PIYEACAEMGLPFAMHLGGQGGVNSTPIGAGPSTFFWETHAILPQSAMTHMASLIAQGVF 238 Query: 205 ARHPNLKMVMLESGFTWLPPFLWRLHKFWRGVRMETPWVDRAPLEIVRSNIRFSLQPVDA 264 + P+LK+V++E G W+P LWRL ++ +R ETPW+ R P E ++NIR S QP++ Sbjct: 239 EKWPSLKVVIIECGVAWVPSVLWRLDANYKALRKETPWLKRLPSEYFKTNIRMSTQPLEQ 298 Query: 265 PPDGDTLNRLFDHMQSDELILFSSDYPHWQFD 296 P + L + M + +LF+SDYPHW +D Sbjct: 299 PENVQHLWATLEAMDGENTLLFASDYPHWDYD 330 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 66.6 bits (161), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 69/236 (29%), Positives = 104/236 (44%), Gaps = 26/236 (11%) Query: 68 GVQMVFSEDMADAFCRALNDWLVKEWLDKDDRLRGSIVIPVQSIEKAVAEIERCAQDKRF 127 GVQ VF ++A R ND L + + DR G + QS + A EIER Q Sbjct: 82 GVQ-VFEPELATTLARQANDQLSEAIKNHPDRFAGLAAVAPQSPQTAALEIERAIQKLGL 140 Query: 128 VQVLMLV-MGDMPLGKRHYWPIYAAAERLGLPVGIHAGSAYHNPPTS--------VGWGS 178 VL+ G L + YW I+AAAE P+ +H + + GWG Sbjct: 141 CGVLINSHTGGEYLDDQKYWEIFAAAEANSAPIYLHPRTPSPEMISPFLDYGLYFAGWG- 199 Query: 179 YHIEDYVAQAQAFQTQLTSLIVEGVFARHPNLKMVMLESGFTWLPPFLWRL-HKFWRGVR 237 + +E T LI+ GVF P L +V+ G LP +L RL +++ V Sbjct: 200 FTVET--------ATHGLRLIMGGVFDHFPKLTIVLGHMG-EGLPYWLQRLDNRYSLQVE 250 Query: 238 M-ETPWVDRAPLEIVRSNIRFSLQPVDAPPDGDTLNRLFDHMQSDELILFSSDYPH 292 + P + R P E + + + V +PP L + + + SD +LF++DYP+ Sbjct: 251 IGAVPKMPRLPSEYFKDHFLITTSGVCSPP---ALRHVLEVLGSDA-VLFAADYPY 302