BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0037.p (369 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the T... 320 3e-87 mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the T... 246 8e-65 SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the T... 202 7e-52 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 101 2e-21 >mlr9222 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 371 Score = 320 bits (820), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 168/366 (45%), Positives = 226/366 (61%), Gaps = 3/366 (0%) Query: 5 LRERPETASPTGVKTAIADCDIHPARATKDELYPYLEKRWHAHLETFGVHAYQGMMEGPP 64 L ++P+ A+P+ T DCDIH + +L +L + W H ET G QG+ + Sbjct: 6 LNQKPKVAAPS--HTGFIDCDIHATWNSLADLDEFLPQYWREHRETIGSRWRQGLAKTMN 63 Query: 65 YPK-AQPNASRRDAYPPEGGPQGSSLSFMQKQHLDPNNVALGVLNPLNTGQGIRNQDLAG 123 YP+ ++ R+DA+P G G+ L MQKQ LD NV G++ RN + Sbjct: 64 YPRMSKGGGVRQDAWPSNGCVPGADLPMMQKQLLDLFNVEYGIMVQPMELSSERNVEFGA 123 Query: 124 AICSAINDWQIEKWTSKDSRLKGSVVVANEDGMSAAAEIRKRAGDKNFVQVLLLSRNVEP 183 A+ +A N+WQ+ W + RLKG+V + ED +A AEI +RAGD+ FVQV R VEP Sbjct: 124 ALATAANEWQLAHWCDPEPRLKGAVQINVEDEKAAIAEIERRAGDRRFVQVQFPQRGVEP 183 Query: 184 LGQRRYWPIYEAAQDAGLPIGVHAFGFGGNPITASGWPSYYIEEMVGHSQCQQTALASLV 243 +G+RRYWPI EA G PI +H G GG+P TA GWPSYY EE + Q Q + SLV Sbjct: 184 IGRRRYWPIMEACAANGFPIAIHIGGIGGHPSTAGGWPSYYHEEHPAYVQAMQNTVISLV 243 Query: 244 LEGVFERFPKLKMVMVEAGFGWAPSLAWRLDKVFSRLHAEVPHLKRKPSEYIRDHIWWTT 303 EGV P LK++++E+GFGW PSL WRLD + RL AEVP L+ KPSEY+R+ IW TT Sbjct: 244 CEGVLSHIPNLKVILLESGFGWLPSLCWRLDTAWKRLRAEVPLLREKPSEYVRERIWLTT 303 Query: 304 QPMEDPESRDHLFQTIEWIGWDRLLFATDYPHWDYDEPSRVLPAGVSDANRQAFYLDNAR 363 QP+++PE L TIEWIG DRL+F+TDYPHWD D+P A + +A ++ + DNAR Sbjct: 304 QPVDEPERPQDLLSTIEWIGEDRLMFSTDYPHWDQDDPRYAFKAALPEAWKRKIFHDNAR 363 Query: 364 QLYGIA 369 LYG++ Sbjct: 364 TLYGLS 369 >mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 357 Score = 246 bits (627), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 138/345 (40%), Positives = 195/345 (56%), Gaps = 5/345 (1%) Query: 23 DCDIHPARATKDELYPYLEKRWHAHLETFGVHAYQGMMEGPPYPKAQPNASRRDAYPPEG 82 DCD+H A L PYL+ W H+ G+ +E YP + P + R D P +G Sbjct: 13 DCDVHVALPDTRALLPYLDDYWREHVIRRGLDRED--LELSSYPPSLPLSCRPDWRPQQG 70 Query: 83 GPQGSSLSFMQKQHLDPNNVALGVLNPLNTGQGIRNQDLAGAICSAINDWQIEKWTSKDS 142 P GS ++ Q LDP + N L+ Q + ++DL+ A+C AIN+W +W +D Sbjct: 71 LP-GSQFEQLRDQVLDPFQSRFAICNVLHGSQIMFSEDLSAALCRAINNWISAEWLDRDP 129 Query: 143 RLKGSVVVANEDGMSAAAEIRKRAGDKNFVQVLLLSRNVEPLGQRRYWPIYEAAQDAGLP 202 RL+ S+VV A AEI RA D FVQVL+L+ PLG+R+ WPIY AA+ LP Sbjct: 130 RLRASIVVPVHSPELAVAEIEHRAADLRFVQVLMLAMGEVPLGRRQNWPIYAAAERHDLP 189 Query: 203 IGVHAFGFGGNPITASGWPSYYIEEMVGHSQCQQTALASLVLEGVFERFPKLKMVMVEAG 262 IG+HA NP + GW S+YI++ V + AL SLV+EGVF +FP+LK+V++E+G Sbjct: 190 IGIHAGSSLRNPPSPLGWSSHYIDDYVSQAPGFSGALNSLVVEGVFNKFPRLKVVLLESG 249 Query: 263 FGWAPSLAWRLDKVFSRLHAEVPHLKRKPSEYIRDHIWWTTQPMEDPESRDHLFQTIEWI 322 W P+ WR +K + + +E P L R PSE IR+H+ T QP + P + + I+ I Sbjct: 250 VTWLPAHLWRANKQWRGVRSETPWLTRPPSEIIREHVRLTVQPFDAPPTDGQVQTIIDQI 309 Query: 323 GWDR-LLFATDYPHWDYDEPSRVLPAGVSDANRQAFYLDNARQLY 366 G D LLF+TDYPHW +D + +P G+S + DNA Y Sbjct: 310 GSDEMLLFSTDYPHWHFDG-TDAVPQGLSHESLSKMLADNALATY 353 >SMa1734 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 366 Score = 202 bits (515), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 125/332 (37%), Positives = 178/332 (53%), Gaps = 13/332 (3%) Query: 21 IADCDIHPARATKDELYPYLEKRWHAHLETFGVHAYQGMMEGPPYPKAQPNA-SRRDAYP 79 I D D H + L PY++ + +G P P SR D P Sbjct: 9 IVDSDCHNYWCSATVLEPYMDGFFKDMFVRGEKTGPRGAFPHGHRPWFHPEGFSRHDVNP 68 Query: 80 PEGGPQGSSLSFMQKQHLDPNNVALGVLN---PLNTGQGIRNQDLAGAICSAINDWQIEK 136 E + + + M+++HLD N+ + +L P+ + N A A+C A ND+ I+ Sbjct: 69 VE---EDDNYAIMKEKHLDKYNIDVAILTGDEPIEAST-LANAHYANALCRAYNDYMIDY 124 Query: 137 WTSKDSRLKGSVVVANEDGMSAAAEIRKRAGDKNFVQVLLLSRNVEPLGQRRYWPIYEAA 196 W KDSR GS++VA +D AA EIR+ VQVL+ P G Y PIYEA Sbjct: 125 WLPKDSRFWGSIIVAPQDPKLAAEEIRRLGSHPRIVQVLVSHGAQRPYGDPFYHPIYEAC 184 Query: 197 QDAGLPIGVHAFGFGG---NPITASGWPSYYIEEMVGHSQCQQTALASLVLEGVFERFPK 253 + GLP +H G GG PI A G +++ E Q T +ASL+ +GVFE++P Sbjct: 185 AEMGLPFAMHLGGQGGVNSTPIGA-GPSTFFWETHAILPQSAMTHMASLIAQGVFEKWPS 243 Query: 254 LKMVMVEAGFGWAPSLAWRLDKVFSRLHAEVPHLKRKPSEYIRDHIWWTTQPMEDPESRD 313 LK+V++E G W PS+ WRLD + L E P LKR PSEY + +I +TQP+E PE+ Sbjct: 244 LKVVIIECGVAWVPSVLWRLDANYKALRKETPWLKRLPSEYFKTNIRMSTQPLEQPENVQ 303 Query: 314 HLFQTIEWI-GWDRLLFATDYPHWDYDEPSRV 344 HL+ T+E + G + LLFA+DYPHWDYD+ +++ Sbjct: 304 HLWATLEAMDGENTLLFASDYPHWDYDDVTKL 335 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 101 bits (252), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 85/284 (29%), Positives = 127/284 (44%), Gaps = 27/284 (9%) Query: 96 HLDPNNVALGVLNPLNTGQGIRNQDLAGAICSAINDWQIEKWTSKDSRLKGSVVVANEDG 155 H+D + + V++ + G + +LA + ND E + R G VA + Sbjct: 65 HMDATGIDIQVISITSPGVQVFEPELATTLARQANDQLSEAIKNHPDRFAGLAAVAPQSP 124 Query: 156 MSAAAEIRKRAGDKNFVQVLLLSRNV-EPLGQRRYWPIYEAAQDAGLPIGVHAFG----- 209 +AA EI + VL+ S E L ++YW I+ AA+ PI +H Sbjct: 125 QTAALEIERAIQKLGLCGVLINSHTGGEYLDDQKYWEIFAAAEANSAPIYLHPRTPSPEM 184 Query: 210 ---FGGNPITASGWPSYYIEEMVGHSQCQQTALASLVLEGVFERFPKLKMVMVEAGFGWA 266 F + +GW G + T L++ GVF+ FPKL +V+ G G Sbjct: 185 ISPFLDYGLYFAGW---------GFTVETATHGLRLIMGGVFDHFPKLTIVLGHMGEGL- 234 Query: 267 PSLAWRLDKVFSRLHAE---VPHLKRKPSEYIRDHIWWTTQPMEDPESRDHLFQTIEWIG 323 P RLD +S L E VP + R PSEY +DH TT + P + H+ +E +G Sbjct: 235 PYWLQRLDNRYS-LQVEIGAVPKMPRLPSEYFKDHFLITTSGVCSPPALRHV---LEVLG 290 Query: 324 WDRLLFATDYPHWDYDEPSRVLP-AGVSDANRQAFYLDNARQLY 366 D +LFA DYP+ +E L A +SD R+ Y NA +L+ Sbjct: 291 SDAVLFAADYPYESVEEAVSFLDNAPISDQERRKIYQTNAEKLF 334