BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0050.p (238 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all3753 [R] COG0300 Short-chain dehydrogenases of various substr... 161 1e-39 SMc01204 [R] COG0300 Short-chain dehydrogenases of various subst... 160 2e-39 CAC2992 [R] COG0300 Short-chain dehydrogenases of various substr... 147 2e-35 PA0658 [R] COG0300 Short-chain dehydrogenases of various substra... 144 2e-34 Rv1544 [R] COG0300 Short-chain dehydrogenases of various substra... 143 3e-34 >all3753 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Nos Nostoc sp. PCC 7120 Length = 263 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 95/242 (39%), Positives = 132/242 (54%), Gaps = 8/242 (3%) Query: 1 VFASNGHRVALVARRADRLKALADEIGAKGGAAPIVIPCDLQHSDAGDRIAQALTDAGVE 60 +FA N + + LV R ++L+ +A E K G + I DL + + + I Q + A ++ Sbjct: 25 IFAENDYNLVLVDRAKEKLEEIAIEFQDKFGISVKPIVRDLSKTTSPEEIFQEVEQANIK 84 Query: 61 VEFLVNNAGFGLFGRAIERDRAEQLNIINVNIRALTDLTLRFADQLIRN-RGGILNLGSI 119 V+ LVNNAGFG +G + + ++L ++ VN+ T LT F +++ G ILN+ S Sbjct: 85 VDVLVNNAGFGTYGLFNDTNLTDELEMLQVNVVCTTHLTKLFLKNMVQQGEGKILNVSSA 144 Query: 120 AGFLPGPGMAVYYASKAYVLSFSEALRCELAPQGVRVTVCCPGPVPSEFQDRAGFAPGFD 179 A F PGP MAVY+A+KAYVLSFSEAL EL GV VTV CPG S F R G A D Sbjct: 145 AAFQPGPLMAVYFATKAYVLSFSEALANELDGTGVTVTVLCPGTTQSAFHQRTGMA---D 201 Query: 180 SAILN----VSPEEVARQAYRGLMANKRAVLPGAFIKLVPFLLRLFPRSFILTAVGGFQL 235 S ++ + VA YRGLM K V+PG K++ +R PR + V Q Sbjct: 202 SKLVKGKRMMDASTVADIGYRGLMQGKTIVIPGLINKIMAKSIRFIPRKLVTKIVRNMQE 261 Query: 236 RK 237 K Sbjct: 262 NK 263 >SMc01204 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Sme Sinorhizobium meliloti Length = 294 Score = 160 bits (405), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 104/243 (42%), Positives = 138/243 (56%), Gaps = 9/243 (3%) Query: 1 VFASNGHRVALVARRADRLKALADEIGAKGGAAPIVIPCDLQHSDAGDRIAQALTDAGVE 60 V A +G V LVAR L A AD+I GG A +P D DA + L+ G+ Sbjct: 27 VAARDGAPVVLVARSEVALAAAADDIRNAGGEA-FTVPLDFLADDAALGLEDFLSANGLF 85 Query: 61 VEFLVNNAGFGLFGRAIERDRAEQLNIINVNIRALTDLTLRFADQLI-RNRGGILNLGSI 119 + LVN+AG+GL G A EQL ++++NIRALT+LTLR ++ R RGG++NLGS+ Sbjct: 86 CDVLVNSAGYGLRGGATVLPLDEQLGLVDLNIRALTELTLRLLPGMVARRRGGVINLGSV 145 Query: 120 AGFLPGPGMAVYYASKAYVLSFSEALRCELAPQGVRVTVCCPGPVPSEFQDRAGFAPGFD 179 A F PGP MA+YYASK +V SFSEAL EL GV VT PGPV +EF +G Sbjct: 146 ASFTPGPYMALYYASKGFVRSFSEALHQELRHTGVTVTCVAPGPVSTEFLATSG---ANR 202 Query: 180 SAILNVSPE----EVARQAYRGLMANKRAVLPGAFIKLVPFLLRLFPRSFILTAVGGFQL 235 A+ + P+ VA +A+RG +R V+PG KL L L P + +L +G Q Sbjct: 203 VALFKILPKLEAGYVAEKAWRGFKTGRRLVIPGLSAKLTILLAALVPSAIMLPLIGRLQR 262 Query: 236 RKR 238 R + Sbjct: 263 RSK 265 >CAC2992 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Cac Clostridium acetobutylicum Length = 262 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 86/238 (36%), Positives = 133/238 (55%), Gaps = 11/238 (4%) Query: 1 VFASNGHRVALVARRADRLKALADEIGAKGGAAPIVIPCDLQHSDAGDRIAQALTDAGVE 60 +FA NG+ V + AR RL LA I K VI DL D+ + + + + +E Sbjct: 19 IFARNGYDVVITARDEKRLIELAKNINTK----VTVIKKDLSRPDSANELFNEIKENNIE 74 Query: 61 VEFLVNNAGFGLFGRAIERDRAEQLN-IINVNIRALTDLTLRFADQLIRNRGG-ILNLGS 118 V+ LVNNAG G G + +++N ++ +NI++LT LT + ++++ R G ILN+ S Sbjct: 75 VDILVNNAGMGDTG-LFHKSSVDKINEMLELNIKSLTILTRLVSAEMVKKRSGMILNVAS 133 Query: 119 IAGFLPGPGMAVYYASKAYVLSFSEALRCELAPQGVRVTVCCPGPVPSEFQDRAGFAPGF 178 ++PGP +AVYYA+KAYVLSF+EA+ EL V+V+ CPG +EF RAG Sbjct: 134 TGSYMPGPFIAVYYATKAYVLSFTEAIGEELKKFNVKVSALCPGATATEFSKRAGKLE-L 192 Query: 179 DSAILNVSPEEVARQAYRGLMANKRAVLPGAFIKLVPFLLRLFPRSFILTAVGGFQLR 236 A+ S + VA AY+ M K+ ++PG F K+ ++ PR+ + +G Q + Sbjct: 193 KGAM---SAKSVAEIAYKEFMNGKKVIIPGCFNKVGILFSKILPRNMMGKIIGKSQKK 247 >PA0658 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Pae Pseudomonas aeruginosa Length = 266 Score = 144 bits (363), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 89/227 (39%), Positives = 125/227 (55%), Gaps = 2/227 (0%) Query: 2 FASNGHRVALVARRADRLKALADEIGAKGGAAPIVIPCDLQHSDAGDRIAQALTDAGVEV 61 A G + LVAR+ D L+++A E+ + G + CDL + L ++ Sbjct: 22 LARRGRALILVARQRDALESIAYELAQRFGVEVLFRTCDLSEPLQLSGLLHELEQGERQI 81 Query: 62 EFLVNNAGFGLFGRAIERDRAEQLNIINVNIRALTDLTLRFADQLIRNRGG-ILNLGSIA 120 + LVNNAG G G I+++ A + +I +NI AL L ++ GG ILN+ S+A Sbjct: 82 DLLVNNAGIGTSGPFIDQEWAREQELIELNILALARLCHSLGARMAAQGGGQILNIASVA 141 Query: 121 GFLPGPGMAVYYASKAYVLSFSEALRCELAPQGVRVTVCCPGPVPSEFQDRAGFAPG-FD 179 F PG M+ YYASKAYVL FSE LR EL +GV+V+V CPGP + F AG G D Sbjct: 142 AFQPGAWMSNYYASKAYVLHFSEGLREELKERGVKVSVLCPGPTRTAFFREAGMRLGRLD 201 Query: 180 SAILNVSPEEVARQAYRGLMANKRAVLPGAFIKLVPFLLRLFPRSFI 226 + L ++PEEVA A RGL N+ ++PG +L+ RL PR + Sbjct: 202 RSKLMMTPEEVALLALRGLDKNRALIVPGWRNRLLALSPRLAPRWLV 248 >Rv1544 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Mtu Mycobacterium tuberculosis H37Rv Length = 267 Score = 143 bits (361), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 96/231 (41%), Positives = 132/231 (57%), Gaps = 6/231 (2%) Query: 2 FASNGHRVALVARRADRLKALADEIGAKGGAAPIVIPCDLQHSDAGDRIAQAL-TDAGVE 60 A G + LVARR +RL LAD++ + V+P DL + A ++A L +DA Sbjct: 29 LAGRGFPLMLVARRRERLDELADQLRQEHCVGVEVLPLDLADTQARAQLADRLRSDA--- 85 Query: 61 VEFLVNNAGFGLFGRAIERDRAEQLNIINVNIRALTDLTLRFADQLI-RNRGGILNLGSI 119 + L N+AGFG GR E A + + +N AL +LT ++ R G +LN+ SI Sbjct: 86 IAGLCNSAGFGTSGRFWELPFARESEEVVLNALALMELTHAALPGMVKRGAGAVLNIASI 145 Query: 120 AGFLPGPGMAVYYASKAYVLSFSEALRCELAPQGVRVTVCCPGPVPSEFQDRAGFAPGFD 179 AGF P P MAVY A+KA+VL+FSEA++ EL GV VT CPGPVP+E+ + A A F Sbjct: 146 AGFQPIPYMAVYSATKAFVLTFSEAVQEELHGTGVSVTALCPGPVPTEWAEIAS-AERFS 204 Query: 180 SAILNVSPEEVARQAYRGLMANKRAVLPGAFIKLVPFLLRLFPRSFILTAV 230 + VSP +VA A G+++ KR V+PG K V R PRS +L A+ Sbjct: 205 IPLAQVSPHDVAEAAIAGMLSGKRTVVPGIVPKFVSTSGRFAPRSLLLPAI 255