BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0049.p (247 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc03236 [F] COG0518 GMP synthase - Glutamine amidotransferase d... 276 3e-74 AGc4818 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 276 3e-74 mll4216 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 251 9e-67 TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 107 2e-23 RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 104 2e-22 >SMc03236 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Sme Sinorhizobium meliloti Length = 252 Score = 276 bits (706), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 130/222 (58%), Positives = 168/222 (75%), Gaps = 2/222 (0%) Query: 11 RPVLIVLHRETSTPGRVGNALRALGYRLDIRRPRFGDALPATLDGHAGAVIFGGPMSAND 70 RP+LIVLH+E S+ GRVG L G+ LDIRRP GD LP TL+ H+G +IFGGPMSAND Sbjct: 14 RPILIVLHQERSSAGRVGQILERKGFSLDIRRPALGDELPPTLEAHSGTIIFGGPMSAND 73 Query: 71 GDDYVRREIDWIAVPLREQRPFLGICLGAQMLARQLGAKVAPHPQGRVEVGYYPVRPTEA 130 +++VRREIDW++VPL E +P+LGICLGAQMLAR LG +V H +G E+G+YP+R T+A Sbjct: 74 EEEFVRREIDWLSVPLLENKPYLGICLGAQMLARNLGGRVEAHHEGMTEIGWYPLRATQA 133 Query: 131 GRALCPDWPARVYHWHGEGFQLPAGADLLASGDDFPVQAFQFG-NAFGLQFHPDVTYAMM 189 G+AL DWPA VYH+H EGF LP GA+LLA+GD +P QAF++G NA+G+QFH ++T AMM Sbjct: 134 GKALM-DWPAMVYHFHREGFDLPKGAELLATGDTYPNQAFRYGENAWGIQFHGELTRAMM 192 Query: 190 HRWTARGCARMDSPGAQPGHLHFAERAVHDAAERAWLKHFID 231 HRW G R + PGAQ G H R +HD R W+++F++ Sbjct: 193 HRWVVHGAHRFELPGAQLGKAHLEGRMLHDHPLRTWMENFLE 234 >AGc4818 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 243 Score = 276 bits (706), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 135/229 (58%), Positives = 164/229 (71%), Gaps = 1/229 (0%) Query: 13 VLIVLHRETSTPGRVGNALRALGYRLDIRRPRFGDALPATLDGHAGAVIFGGPMSANDGD 72 +L+VLH+E STPGRVG L GYRLDIRRP GD LP TL+ HAGA+IFGGPMSAND Sbjct: 15 LLVVLHQERSTPGRVGQILVEKGYRLDIRRPALGDDLPQTLERHAGAIIFGGPMSANDPH 74 Query: 73 DYVRREIDWIAVPLREQRPFLGICLGAQMLARQLGAKVAPHPQGRVEVGYYPVRPTEAGR 132 DYV+ EIDW+ VPL+E +PFLGICLGAQML++ LG KV G+VE+G+YP+ TE GR Sbjct: 75 DYVKAEIDWLKVPLKENKPFLGICLGAQMLSKHLGGKVEADRDGKVEIGWYPLHATEHGR 134 Query: 133 ALCPDWPARVYHWHGEGFQLPAGADLLASGDDFPVQAFQFG-NAFGLQFHPDVTYAMMHR 191 L P WP VYH+H EGF+LP GA+LLASG+ +P QA+++G NA+GLQFH ++T AMMH Sbjct: 135 LLMPHWPKMVYHFHKEGFELPRGAELLASGETYPNQAYRYGKNAWGLQFHAELTRAMMHS 194 Query: 192 WTARGCARMDSPGAQPGHLHFAERAVHDAAERAWLKHFIDGWIARVPQR 240 W RG R P AQ G H R + D RAWL +F+D PQR Sbjct: 195 WVVRGAQRFGMPNAQVGSQHLEGRMLFDTPLRAWLGNFLDLVFEGKPQR 243 >mll4216 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Mlo Mesorhizobium loti Length = 225 Score = 251 bits (642), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 123/216 (56%), Positives = 153/216 (70%), Gaps = 2/216 (0%) Query: 16 VLHRETSTPGRVGNALRALGYRLDIRRPRFGDALPATLDGHAGAVIFGGPMSANDGDDYV 75 +LH+E S+PGRVG+ L G+ LDIRRP GDALP TLDGHAG V+FGGPMSAND D++V Sbjct: 1 MLHQENSSPGRVGHMLIEEGFELDIRRPPLGDALPETLDGHAGTVVFGGPMSANDEDEFV 60 Query: 76 RREIDWIAVPLREQRPFLGICLGAQMLARQLGAKVAPHPQGRVEVGYYPVRPTEAGRALC 135 RRE +W+ +PLRE RP LGICLGAQML LG KV H +G VE+G+YP++ TEAG+ L Sbjct: 61 RRETNWLEIPLRENRPCLGICLGAQMLVNHLGGKVEGHGEGLVEIGWYPLKATEAGKKLM 120 Query: 136 PDWPARVYHWHGEGFQLPAGADLLASGDDFPVQAFQFG-NAFGLQFHPDVTYAMMHRWTA 194 WP VY +H EGF LP A LLA+ D +P QAF++G NA+G+QFH ++T MM RW Sbjct: 121 -HWPDMVYQFHREGFSLPRDATLLATADTYPNQAFRYGDNAWGIQFHGELTRVMMQRWVV 179 Query: 195 RGCARMDSPGAQPGHLHFAERAVHDAAERAWLKHFI 230 RG R + PGAQPG H R + D + WL F+ Sbjct: 180 RGAHRFELPGAQPGRDHLGGRLIWDMHLKRWLDEFL 215 >TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Tma Thermotoga maritima Length = 227 Score = 107 bits (268), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 69/215 (32%), Positives = 102/215 (47%), Gaps = 13/215 (6%) Query: 13 VLIVLHRETSTPGRVGNALRALGYRLDIRRPRFGDALPATLDGHAGAVIFGGPMSANDGD 72 VL + H E G + + R + D G+ L L+ ++ V+ GG M A + + Sbjct: 3 VLAIRHVEIEDLGMMEDIFREKNWSFDYLDTPKGEKLERPLEEYSLVVLLGGYMGAYEEE 62 Query: 73 DY--VRREIDWIAVPLREQRPFLGICLGAQMLARQLGAKVAPHPQGRVEVGYYPVRPTEA 130 Y ++ E I L+++ PFLGICLG+QMLA+ LGA V G E+G+Y V Sbjct: 63 KYPFLKYEFQLIEEILKKEIPFLGICLGSQMLAKVLGASVYRGKNGE-EIGWYFVEKVSD 121 Query: 131 GRAL--CPDWPARVYHWHGEGFQLPAGADLLASGDDFPVQAFQFGNAFGLQFHPDVTYAM 188 + PD RV+ WHG+ F LP A + + + + Q F +G A GLQFH +V Sbjct: 122 NKFFREFPD-RLRVFQWHGDTFDLPRRATRVFTSEKYENQGFVYGKAVGLQFHIEVGART 180 Query: 189 MHRWTARGCARMDSPGAQPGHLHFAERAVHDAAER 223 M RW ++ P R + + AER Sbjct: 181 MKRWIEAYKDELEKKKIDP-------RLLLETAER 208 >RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Rso Ralstonia solanacearum Length = 242 Score = 104 bits (259), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 66/155 (42%), Positives = 83/155 (53%), Gaps = 8/155 (5%) Query: 60 VIFGGPMSANDGDDY--VRREIDWIAVPLREQRPFLGICLGAQMLARQLGAKVAPHPQGR 117 V+ GGP+ A D D Y +R E D IA L ++P LGICLGAQ++AR LGA VAP Sbjct: 51 VVLGGPIGAFDDDRYPFLREETDAIARRLAARKPLLGICLGAQLMARALGAAVAPMGDNV 110 Query: 118 VEVGYYPVRPTEAGRALCPDWP----ARVYHWHGEGFQLPAGADLLASGDDFPVQAFQFG 173 E+G+ P+ T G A P P V HWHG+ F+LP GA LAS QAF G Sbjct: 111 KEIGFAPLTLTAEGAA-SPLAPLADGTPVLHWHGDRFELPPGATRLASTSVCAEQAFALG 169 Query: 174 N-AFGLQFHPDVTYAMMHRWTARGCARMDSPGAQP 207 + A GLQFH + + W A + + G P Sbjct: 170 DHALGLQFHLEADLRELEAWLIGHAAELAAAGIDP 204