FastQCFastQC Report
Sat 26 Oct 2024
IN5-30_23_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN5-30_23_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69366
Sequences flagged as poor quality0
Sequence length35-251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG8561.2340339647666003No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA6370.9183173312573882No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC5950.8577689357898682No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC4820.6948649194129689No Hit
CGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA4370.6299916385549116No Hit
CGTAGGTCACGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGG4260.6141337254562754No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG2460.35464060202404635No Hit
CGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGG2080.2998587204105758No Hit
CGTAGGTCACGAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGG2070.2984170919470634No Hit
GTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC1950.2811175503849148No Hit
GTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC1650.23786869647954328No Hit
AGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTG1450.2090361272092956No Hit
CGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGG1420.20471124181875847No Hit
GTAGGTGGCAAGCGTTATCGGGATTTATTGGGCGTAAAGCGAGCGCAGGC1410.20326961335524607No Hit
CGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGG1370.19750309950119657No Hit
ACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAG1280.1845284433295851No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTAGTGGGCGTAAAGCGAGCGCAGG1270.18308681486607273No Hit
GTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC1260.18164518640256033No Hit
CGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAGG1250.18020355793904794No Hit
GTAGGTCACGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGC1240.17876192947553557No Hit
CGTAGGTGGCAAGCGTTATCCGGAGTTATTGGGCGTAAAGCGAGCGCAGG1190.17155378715797365No Hit
CGTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGA1130.16290401637689933No Hit
GTAGGTGGCAAGCGTGATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC1120.16146238791338696No Hit
CGTAGGTGGCAAGCGTTATCGGGATTTATTGGGCGTAAAGCGAGCGCAGG1060.15281261713231264No Hit
GTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGTGTAGGC1030.1484877317417755No Hit
GTAGGTGGCAAGCGTTATCCGGATTTAGTGGGCGTAAAGCGAGCGCAGGC1030.1484877317417755No Hit
GTAGGTGGCAAGCGTTATCCGGAGTTATTGGGCGTAAAGCGAGCGCAGGC1010.14560447481475072No Hit
CGTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGA990.142721217887726No Hit
CGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA980.1412795894242136No Hit
GTAGGTCACGAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGGC950.13695470403367643No Hit
CGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGG950.13695470403367643No Hit
GTAGGTGGCAAGCGTTATCCGGATGTATTGGGCGTAAAGCGAGCGCAGGC910.1311881901796269No Hit
GTAGGGGGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGAC910.1311881901796269No Hit
GTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGC900.1297465617161145No Hit
CGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGA890.12830493325260214No Hit
GTAGGGGGCAAGCGTTATCCGGATTGACTGGGTGTAAAGGGAGCGTAGAC830.11965516247152783No Hit
CGTAGGTCACGAGCGTTGTTCGGAATTACTGGGTGTAAAGGGAGCGCAGG820.11821353400801546No Hit
CGTAGGTGGCAAGCGTGATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG820.11821353400801546No Hit
CGTAGGTGGCAAGCGTTATCCGGATGTATTGGGCGTAAAGCGAGCGCAGG820.11821353400801546No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG800.1153302770809907No Hit
CGTAGGGGGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGA740.10668050629991639No Hit
GTAGGTGGCAAGCGTTATCCGGATTGATTGGGCGTAAAGCGAGCGCAGGC740.10668050629991639No Hit
GTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGC720.10379724937289161No Hit
CGTAGGTGGCAAGCGTTATCCGGATTGATTGGGCGTAAAGCGAGCGCAGG710.10235562090937923No Hit
CGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGG700.10091399244586685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)