FastQCFastQC Report
Sat 26 Oct 2024
IN5-30_23_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN5-30_23_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69366
Sequences flagged as poor quality0
Sequence length247-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG55598.01401262866534No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG37245.368624398120116No Hit
CTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGAAC18582.678545685206009No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA15752.2705648300320043No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTAAG14432.0802698728483695No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG13601.9606147103768419No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC10141.4618112620015569No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG9611.3854049534354005No Hit
TGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG9271.3363895856759795No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC6410.9240838451114378No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG6240.8995761612317273No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG6180.8909263904506531No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG5760.8303779949831329No Hit
TGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAG5650.8145200818844968No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG3780.544935559207681No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAG3380.4872704206671856No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAGGCCCAGCAGG3180.458437851396938No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG3060.44113830983478935No Hit
TGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG3030.4368134244442522No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG2990.43104691059020267No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGCAAG2890.4166306259550789No Hit
CTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGACC2870.4137473690280541No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAG2790.402214341319955No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAGG2770.39933108439293025No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG2700.38923968514834356No Hit
GTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGC2630.37914828590375693No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA2600.3748234005132197No Hit
CTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCGGTGGCGGCCCAGAGG2320.334457803534873No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG2190.31571663350921203No Hit
CTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAA2180.31427500504569966No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGATGGCCCAGCAA2180.31427500504569966No Hit
CTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA2080.2998587204105758No Hit
TGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAG2040.29409220655652624No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTAAG2040.29409220655652624No Hit
CTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTAG2020.29120894962950145No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTAAG1960.2825591788484272No Hit
TGTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGACAG1940.27967592192140245No Hit
CTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGACC1900.27390940806735287No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAG1780.2566098665052043No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAG1700.24507683879710523No Hit
GTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGC1630.23498543955251852No Hit
TGGTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTAAG1620.23354381108900615No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAG1610.23210218262549376No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG1600.2306605541619814No Hit
TGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAG1500.21624426952685755No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGGAAG1460.21047775567280802No Hit
GGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC1390.20038635642822133No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT1370.19750309950119657No Hit
TGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGG1360.19606147103768418No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG1360.19606147103768418No Hit
TGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG1360.19606147103768418No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1300.18741170025660986No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAAAG1240.17876192947553557No Hit
GGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC1220.1758786725485108No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGC1200.17299541562148604No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCAAG1160.1672289017674365No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG1110.16002075944987457No Hit
GTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTAAGC1060.15281261713231264No Hit
TGTTTGCTCCCCCCGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG1000.14416284635123836No Hit
TGGTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG1000.14416284635123836No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAG1000.14416284635123836No Hit
TGTTTGCGCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG990.142721217887726No Hit
GTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC980.1412795894242136No Hit
TGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAG960.1383963324971888No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGG950.13695470403367643No Hit
CTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAAA950.13695470403367643No Hit
TGTTTGCTACCCACACTTCCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG940.13551307557016407No Hit
TGTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG910.1311881901796269No Hit
GTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGC900.1297465617161145No Hit
CTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAA900.1297465617161145No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAAG900.1297465617161145No Hit
TGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG890.12830493325260214No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGAG890.12830493325260214No Hit
TGGTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAG870.12542167632557738No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCAAT860.123980047862065No Hit
TGTTCGCTACCCAGGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG840.12109679093504021No Hit
TGTTCGCTACCCATGCTTCCGAGCCTCAGCGTCAGTTGCAGACCAGACAG820.11821353400801546No Hit
TGTGTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG800.1153302770809907No Hit
TGGTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG770.11100539169045354No Hit
CTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGATGGCCCAGCAA750.10812213476342877No Hit
TGTTCGCTACCCATGCTTTCGAGCCGCAGCGTCAGTTGCAGACCAGACAG730.10523887783640401No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGG720.10379724937289161No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCAGG720.10379724937289161No Hit
TGTTTGCTCCCCACGCTTTCGAGCCGCAACGTCAGTTACCGTCCAGTAAG710.10235562090937923No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAG710.10235562090937923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)