FastQCFastQC Report
Sat 26 Oct 2024
IN4-50_26_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN4-50_26_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62759
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG8401.3384534489077262No Hit
CGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA7531.1998279131279976No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA5870.9353240172724231No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC5300.844500390382256No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC5040.8030720693446357No Hit
CGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGG4650.7409295877882057No Hit
GTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC3580.5704361127487692No Hit
GTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGC2240.356920919708727No Hit
CGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGG1950.31071240778215076No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGG1820.28999824726334067No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG1790.2852180563743846No Hit
CGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGA1720.27406427763348684No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG1610.2565369110406476No Hit
CGTAGGTCACGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGG1610.2565369110406476No Hit
CGTAGGTGGCAAGCGTTATCCGGAGTTATTGGGCGTAAAGCGAGCGCAGG1520.24219633837377907No Hit
CGTAGGTGGCAAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGG1460.23263595659586672No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTAGTGGGCGTAAAGCGAGCGCAGG1380.2198887808919836No Hit
GTAGGTGGCAAGCGTTATCCGGAGTTATTGGGCGTAAAGCGAGCGCAGGC1330.2119217960770567No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGC1330.2119217960770567No Hit
GTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC1240.1975812234101882No Hit
CGTAGGATGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCAGG1130.18005385681734892No Hit
CGTATGGTGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGA1130.18005385681734892No Hit
GTAGGTGGCAAGCGTGATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC1090.17368026896540736No Hit
GTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGGC1070.17049347503943657No Hit
GTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC1060.1689000780764512No Hit
GTAGGTGGCAAGCGTTATCCGGATTTAGTGGGCGTAAAGCGAGCGCAGGC1050.16730668111346578No Hit
GTAGGTGGCAAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGGC1020.16252649022450963No Hit
ACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAG980.15615290237256807No Hit
CGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCAGG980.15615290237256807No Hit
GTAGGTGGCAAGCGTTATCGGGATTTATTGGGCGTAAAGCGAGCGCAGGC980.15615290237256807No Hit
GTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTAGGC940.14977931452062654No Hit
GTAGGTGGCAAGCGTTATCCGGATGTATTGGGCGTAAAGCGAGCGCAGGC880.1402189327427142No Hit
ACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAG880.1402189327427142No Hit
CGTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGA850.13543874185375804No Hit
GTAGGTGGCAAGCGTTATCCGGATTGATTGGGCGTAAAGCGAGCGCAGGC850.13543874185375804No Hit
GTAGGATGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCAGGC840.13384534489077263No Hit
CGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA830.13225194792778724No Hit
GTAGGGGGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGAC830.13225194792778724No Hit
CGTAGGTGGCAAGCGTGATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG820.13065855096480186No Hit
CGTATGGTGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGA810.12906515400181648No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC800.1274717570388311No Hit
GTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCAGGC790.12587836007584569No Hit
CGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAGG780.1242849631128603No Hit
GTAGGGGGCAAGCGTTATCCGGATTGACTGGGTGTAAAGGGAGCGTAGAC780.1242849631128603No Hit
CGTAGGTGGCAAGCGTTATCGGGATTTATTGGGCGTAAAGCGAGCGCAGG760.12109816918688954No Hit
CGTAGGTGGCAAGCGTTATCCGGATGTATTGGGCGTAAAGCGAGCGCAGG740.11791137526091874No Hit
CGTATGGTGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGA740.11791137526091874No Hit
GTATGGTGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC730.11631797829793336No Hit
CGTATGGTGCAAGCGTTATCCGGATTGACTGGGTGTAAAGGGAGCGTAGA700.1115377874089772No Hit
GTATGGTGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC690.1099443904459918No Hit
CGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGG690.1099443904459918No Hit
GTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC690.1099443904459918No Hit
GTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGTGTAGGC690.1099443904459918No Hit
GTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTAGGC670.10675759652002104No Hit
ACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCAG670.10675759652002104No Hit
CGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAGG660.10516419955703565No Hit
GTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGC660.10516419955703565No Hit
CGTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGA650.10357080259405026No Hit
GTAGGTCACGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGC640.10197740563106486No Hit
GTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGAC630.10038400866807946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)