FastQCFastQC Report
Sat 26 Oct 2024
IN4-50_26_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN4-50_26_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62759
Sequences flagged as poor quality0
Sequence length248-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG34055.425516658965248No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG33275.301231695852388No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG23053.6727799996813206No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG15842.5239407893688557No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA10011.594990359948374No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAG9971.5886167720964322No Hit
CTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGACC9231.4707053968355137No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC8861.4117497092050542No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG7961.2683439825363694No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG6681.0643891712742395No Hit
CTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGACC6391.0181806593476632No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAG6090.9703787504581016No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG5790.92257684156854No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTAAG5740.9146098567536131No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG4940.787138099714782No Hit
CTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA4360.6947210758616295No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGCAAG4300.685160694083717No Hit
CTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGAAC4100.6532927548240093No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGG3820.6086776398604183No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAAAG3810.6070842428974331No Hit
CTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGTCCAGATC3690.5879634793416083No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC3570.5688427157857837No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG3490.5560955400819005No Hit
TGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAG3300.5258209977851782No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG2940.4684587071177042No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGG2690.4286237830430696No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG2610.4158766073391864No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAG2580.4110964164502302No Hit
TGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAG2550.40631622556127406No Hit
TGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAAAG2490.39675584378336176No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG2430.3871954620054494No Hit
TGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAG2410.38400866807947864No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG2360.37604168326455173No Hit
TGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG2340.3728548893385809No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG2310.36807469844962476No Hit
TGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTGATGGCCCAGAAG2190.34895393489380006No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAAAG2050.32664637741200464No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAGG1930.30752561385618No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGC1900.30274542296722384No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAGGCCCAGCAGG1810.28840485030035534No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCAAT1730.2756576745964722No Hit
TGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG1690.2692840867445307No Hit
CTATTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTAATGGCCCAGCAA1680.26769068978154525No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT1610.2565369110406476No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAAG1610.2565369110406476No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAG1570.250163323188706No Hit
TGGTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG1570.250163323188706No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA1560.2485699262257206No Hit
TGTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGACAG1540.2453831322997498No Hit
TGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAG1440.22944916266989598No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAATGTCAGTTACTGTCCAGAAAG1430.22785576570691057No Hit
GTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGC1340.21351519304004207No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC1320.2103283991140713No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAG1240.1975812234101882No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAG1230.1959878264472028No Hit
CTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAA1160.18483404770630507No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTAAG1140.18164725378033428No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCAAG1130.18005385681734892No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAG1130.18005385681734892No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAG1070.17049347503943657No Hit
TGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGG1040.16571328415048042No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGGCCCAGCAA1010.16093309326152425No Hit
GGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC980.15615290237256807No Hit
TGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGG970.1545595054095827No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAGC970.1545595054095827No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGG950.15137271148361192No Hit
GTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGC890.1418123297056996No Hit
TGTTCGCTACCCAGGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG880.1402189327427142No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG840.13384534489077263No Hit
GGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC830.13225194792778724No Hit
TGTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG780.1242849631128603No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACTGTCCAGTAAG770.1226915661498749No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG770.1226915661498749No Hit
GTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC770.1226915661498749No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGG760.12109816918688954No Hit
TGTTCGCTACCCATGCTTCCGAGCCTCAGCGTCAGTTGCAGACCAGACAG740.11791137526091874No Hit
TATTTGCTCCCCACGCTTTCGGGACTCAGCGTCTGTTTCAGCCCAGATCG730.11631797829793336No Hit
TGGTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTAAG730.11631797829793336No Hit
TGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGCCCAGTAAG730.11631797829793336No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGC720.11472458133494799No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG710.1131311843719626No Hit
CGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAAAG710.1131311843719626No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAAAG690.1099443904459918No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTTAT690.1099443904459918No Hit
TGTTTGCTCCCCCCGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG690.1099443904459918No Hit
CTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAAA690.1099443904459918No Hit
TGGTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG690.1099443904459918No Hit
TTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCC680.10835099348300642No Hit
GTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC680.10835099348300642No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGC680.10835099348300642No Hit
GTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGC670.10675759652002104No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACTGTCCAGTAAG670.10675759652002104No Hit
CTAGTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGACC660.10516419955703565No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG660.10516419955703565No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAG630.10038400866807946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)