FastQCFastQC Report
Sat 26 Oct 2024
IN3-50_27_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN3-50_27_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54164
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA7481.380991064175467No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA5180.956354774388893No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC4780.8825049848607932No Hit
ACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAG4700.8677350269551732No Hit
GTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC3150.5815670925337862No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG3130.5778746030573813No Hit
CGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGG3130.5778746030573813No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG2220.4098663318809541No Hit
CGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGG1640.30278413706520935No Hit
CGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGA1600.2953991581123994No Hit
CGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAGG1340.2473967949191345No Hit
ACGTAGGTCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAG1280.2363193264899195No Hit
CGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG1270.234473081751717No Hit
GTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGC1260.2326268370135145No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG1190.21970312384609705No Hit
GTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC1170.21601063436969206No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC1170.21601063436969206No Hit
CGTAGGTGGCAAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGG1150.21231814489328704No Hit
GTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC1130.20862565541688205No Hit
CGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCAGG1070.1975481869876671No Hit
CGTATGGTGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGA1010.1864707185584521No Hit
CGTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGA1000.1846244738202496No Hit
GTAGGGGGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGAC870.16062329222361718No Hit
ACGTAGGTCCCGAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGCGCAG850.15693080274721216No Hit
GTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTAGAC820.15139206853260467No Hit
GTAGGGGGCAAGCGTTATCCGGATTGACTGGGTGTAAAGGGAGCGTAGAC780.1440070895797947No Hit
GTAGGTGGCAAGCGTTGTCGGGAATTACTGGGTGTAAAGGGAGCGCAGGC760.1403146001033897No Hit
GTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCAGGC730.13477586588878224No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGG710.13108337641237722No Hit
GTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGC680.12554464219776973No Hit
GTATGGTGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC680.12554464219776973No Hit
CGTATGGTGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGA680.12554464219776973No Hit
GTATGGTGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC660.12185215272136475No Hit
ACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCAG640.11815966324495975No Hit
CGTATGGTGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGA640.11815966324495975No Hit
ACGTAGGTCCCGAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGCGCAG630.11631341850675725No Hit
ACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAG610.11262092903035227No Hit
GTATGGTGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGAC610.11262092903035227No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC610.11262092903035227No Hit
CGTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGA610.11262092903035227No Hit
CGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTAGG570.10523595007754227No Hit
ACGTAGGTGCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAG550.10154346060113728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)