FastQCFastQC Report
Sat 26 Oct 2024
IN3-50_27_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN3-50_27_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54164
Sequences flagged as poor quality0
Sequence length247-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG36386.716638357580681No Hit
CTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA26904.9663983457647145No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG23534.344213868990473No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA19143.5337124289195776No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAG15752.907835462668931No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG10481.934864485636216No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG9881.824089801344066No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG7841.447455874750757No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG6781.2517539325012923No Hit
CTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGACC5871.0837456613248653No Hit
CTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGAAC5731.05789823499003No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGG5060.934199837530463No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGCAAG4420.8160401742855032No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAG4140.7643453216158334No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGG3950.729266671589986No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAAAG3930.725574182113581No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC3630.670186839967506No Hit
CTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGACC3610.6664943504911011No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAAAG3450.6369544346798612No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG3280.6055682741304187No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC2920.539103463555129No Hit
CTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGTCCAGATC2890.5335647293405213No Hit
CTGGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA2840.5243335056495089No Hit
TGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAG2830.5224872609113064No Hit
CTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCGGTGGCGGCCCAGAGG2600.480023631932649No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA2470.4560224503360165No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTAAG2380.4394062476921941No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG2380.4394062476921941No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAG2370.4375600029539916No Hit
CGGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA2280.4209438003101691No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG2210.4080200871427516No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAG1960.3618639686876893No Hit
TGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGG1960.3618639686876893No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT1860.3434015213056643No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG1810.3341702976146518No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGC1780.3286315634000443No Hit
CTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTAG1710.3157078502326269No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAAG1650.30463038180341184No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAG1570.2898604238977919No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTAAGC1390.25662801861014695No Hit
GTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGC1340.2473967949191345No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAGGCCCAGCAGG1340.2473967949191345No Hit
TGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG1310.241858060704527No Hit
TGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG1290.23816557122812199No Hit
GTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGC1270.234473081751717No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTAAG1190.21970312384609705No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAG1180.21785687910789456No Hit
TGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTGATGGCCCAGAAG1180.21785687910789456No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG1130.20862565541688205No Hit
TGGTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGG1020.1883169632966546No Hit
TGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG980.18093198434384464No Hit
CTGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTACAGACCAGAGA960.17723949486743962No Hit
GTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGC940.17354700539103463No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC900.16616202643822464No Hit
CTGTTTGCTCCCCCCGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA890.16431578170002215No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG880.16246953696181965No Hit
CTGTTTGCTCCCCACGCTGTCGAGCCTCAGCGTCAGTTACAGACCAGAGA860.15877704748541469No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG830.15323831327080717No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGAG830.15323831327080717No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCAGG810.14954582379440218No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGC750.1384683553651872No Hit
GGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGC750.1384683553651872No Hit
TGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAAAG720.1329296211505797No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAG690.12739088693597223No Hit
TGGTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG680.12554464219776973No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG670.12369839745956725No Hit
CTGTGTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGA660.12185215272136475No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACTGTCCAGTAAG660.12185215272136475No Hit
CTGTTTGCTCCCCACGCTGTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA650.12000590798316225No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACTGTCCAGTAAG650.12000590798316225No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG640.11815966324495975No Hit
TGTTTGCTCCCCCCGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTAAG600.11077468429214977No Hit
CTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAAA600.11077468429214977No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAAAG590.10892843955394728No Hit
TGTTTGCTCCCCACACTTGCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG570.10523595007754227No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTCACCGTCCAGTAAG570.10523595007754227No Hit
TGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGG560.10338970533933978No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAG560.10338970533933978No Hit
CTGTGTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA550.10154346060113728No Hit
TGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAG550.10154346060113728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)