FastQCFastQC Report
Sat 26 Oct 2024
IN16-50_46_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN16-50_46_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43977
Sequences flagged as poor quality0
Sequence length35-251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGG3870.8800054573981855No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG3330.7572139982263456No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA3320.7549400823157559No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC3170.7208313436569116No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGC2920.663983445892171No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC1860.42294835936967046No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGC1710.3888396207108261No Hit
GTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGC1680.3820178729790572No Hit
GTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAGGC1650.37519612524728835No Hit
GTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGC1520.3456352184096232No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGG1430.32516997521431656No Hit
CGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAGG1430.32516997521431656No Hit
CGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGG1300.29560906837665146No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG1290.29333515246606184No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGG1240.2819655729131137No Hit
ACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAG1090.24785683425426924No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGG1030.23421333879073153No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGG920.20920026377424564No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGGC870.19783068422129751No Hit
GTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGTGCAGGC870.19783068422129751No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGCGCAGG840.1910089364895286No Hit
GTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC790.17963935693658048No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGC740.16826977738363236No Hit
GTAGGTGGCAAGCGTTGTCGGGATTTATTGGGCGTAAAGCGAGTGCAGGC710.16144802965186347No Hit
GTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGTGCAGGC700.15917411374127385No Hit
GGAGGGGGCGAGCGTTGTTCGGAGTGACTGGGCGTAAAGAGCACGTAGGC680.1546262819200946No Hit
GTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGTGCAGGC610.1387088705459672No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGC600.13643495463537758No Hit
CGTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGTGCAGG590.13416103872478796No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCC570.12961320690360872No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGG560.12733929099301908No Hit
ACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAG560.12733929099301908No Hit
GTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGCGCAGGC540.12279145917183984No Hit
CGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAGG530.12051754326125019No Hit
GTAGGGGGCAAGCGTTATCCGGAGTTACTGGGTGTAAAGGGAGCGTAGAC500.11369579552948132No Hit
GTAGGTGGCGAGCGTTATCGGGAATGATTGGGCGTAAAGGGTGCGCAGGC500.11369579552948132No Hit
GTAGGTGGCGAGCGTTGTCGGGATTTACTGGGCGTAAAGGGAGCGTAGGC500.11369579552948132No Hit
CGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGG490.1114218796188917No Hit
CGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCTCGTAGG480.10914796370830207No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGCGCAGGC480.10914796370830207No Hit
CGTAGGTGGCGAGCGTTATCGGGAATGATTGGGCGTAAAGGGTGCGCAGG470.10687404779771244No Hit
GTAGGGGGCAAGCGTTATCCGGATGTACTGGGTGTAAAGGGAGCGTAGAC470.10687404779771244No Hit
GGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCTCGTAGGT460.10460013188712282No Hit
CGTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGTGCAGG450.10232621597653319No Hit
CGTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGCGCAGG440.10005230006594355No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG440.10005230006594355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)