FastQCFastQC Report
Sat 26 Oct 2024
IN16-50_46_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN16-50_46_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43977
Sequences flagged as poor quality0
Sequence length247-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG23675.382358960365646No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA12052.7400686722604997No Hit
TATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG11882.7014121017804764No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG10302.342133387907315No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT9302.1147417968483526No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA8681.9737590103917957No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG7571.7213543443163473No Hit
TGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG6771.539441071469177No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGA5751.3075016485890352No Hit
TGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAGAC4180.9504968506264638No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC3140.7140095959251427No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTAT2860.6503399504286331No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG2700.6139572958591991No Hit
TGTTTGCTACCCACGCTTTCGCTCCTCAGTGTCAGTTATGTGCCAGATAG2550.5798485572003547No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAACAGTCCAGGCG2530.5753007253791755No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGTAT2360.5366441548991518No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCTCAGTCCAGCAGG2330.5298224071673829No Hit
TGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTTGCAGTCCAGCAGG1980.450235350296746No Hit
TGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGTGG1910.43431793892261866No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTAAC1860.42294835936967046No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAG1830.4161266116379016No Hit
TGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTTCCAGCCAGATTG1740.39566136844259503No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1690.3842917888896469No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCAGG1680.3820178729790572No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG1600.36382654569434025No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG1600.36382654569434025No Hit
CTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGATGGCCCAGAAG1590.3615526297837506No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTCAT1500.34108738658844395No Hit
CTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAAG1330.3024308161084203No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA1290.29333515246606184No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGG1290.29333515246606184No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAGG1240.2819655729131137No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGGCCAGGCG1220.2774177410919344No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGG1120.25467858198603816No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG1110.25240466607544854No Hit
TGTTCGCTCCCCGCACCTTCGCACCTCAGCGTCAGTCTTCTGCCGGCAAC1100.2501307501648589No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG1100.2501307501648589No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA1090.24785683425426924No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAG1070.24330900243309003No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCAGA1070.24330900243309003No Hit
TGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG1040.23648725470132112No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTAACAGTCCAGGCG1030.23421333879073153No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCAGG1020.2319394228801419No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCCGTCCAGCAGG1010.22966550696955226No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGCAGG940.21374809559542488No Hit
CCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGATTCGGTCCGGAAA940.21374809559542488No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCAGG920.20920026377424564No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGC920.20920026377424564No Hit
TGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAG890.20237851604247675No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTGGGCCAGAAAG890.20237851604247675No Hit
TGTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG880.2001046001318871No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT870.19783068422129751No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTAAG840.1910089364895286No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAG840.1910089364895286No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAG830.18873502057893898No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATC780.17736544102599086No Hit
TGGTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG770.17509152511540124No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGAAG770.17509152511540124No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGCAAG760.1728176092048116No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTAAG730.16599586147304274No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACGGTCCAGCAGG730.16599586147304274No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAG660.15007845009891535No Hit
TGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATGTTCCAGTAGG660.15007845009891535No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATTGTCCAGTAAG650.14780453418832573No Hit
TGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAG630.14325670236714647No Hit
TAGTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG610.1387088705459672No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAG610.1387088705459672No Hit
TGTTCGCTACCCATGCTTCCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG610.1387088705459672No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGG600.13643495463537758No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTGAG600.13643495463537758No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTAAG600.13643495463537758No Hit
GGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC600.13643495463537758No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGAC600.13643495463537758No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGG580.13188712281419832No Hit
TGTTTGCTCCCCGCACCTTCGCACCTCAGCGTCAGTTATCTGCTTGAAAC580.13188712281419832No Hit
TATTTGCTCCCCACGCTTGCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG570.12961320690360872No Hit
TGTTTGCTCCCCACGCTTTCGCGCATCAGCGTCAGTTATGTTCCAGCAGG570.12961320690360872No Hit
CTGGTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTAT550.12506537508242946No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTTAT540.12279145917183984No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAAT540.12279145917183984No Hit
CTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAGA530.12051754326125019No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTAT530.12051754326125019No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGA530.12051754326125019No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG520.11824362735066056No Hit
GTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAGC520.11824362735066056No Hit
TGTTCGCTCCCCGCGCCTTCGCGCCTCAGCGTCAGTCGTCCGCTGGAAGC520.11824362735066056No Hit
CTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAATAGCACCCCAGGAG500.11369579552948132No Hit
TGTTTGATCCCCACGCTTTCGTGCCTGAGCGTCATCCATGGCCTCGTGAG480.10914796370830207No Hit
TGTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG470.10687404779771244No Hit
GTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC470.10687404779771244No Hit
CTAGTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG470.10687404779771244No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTAAG460.10460013188712282No Hit
TTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTTCTGTCCAGTAAGCC450.10232621597653319No Hit
CTGTTTGCTCCCCACGCTGTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA450.10232621597653319No Hit
TGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAAG440.10005230006594355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)