FastQCFastQC Report
Sat 26 Oct 2024
IN15-50_43_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN15-50_43_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67846
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGG6190.9123603454883118No Hit
GTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGC6110.900568935530466No Hit
CGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGG6080.896147156796274No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG5800.8548772219438139No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG5370.7914983934203932No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGC4650.6853757037997819No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC3210.4731303245585591No Hit
CGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAGG2800.4126993485245999No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGG2070.3051027326592577No Hit
CGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGG1840.2712024290304513No Hit
CGTAGGATGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGG1600.23582819915691416No Hit
GTAGGTGGCGAGCGTTGTCGGGATTTACTGGGCGTAAAGGGAGCGTAGGC1590.23435427291218347No Hit
ACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAG1560.22993249417799133No Hit
GTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCAGGT1290.19013648557026208No Hit
GTAGGTGGCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGGGAGCGTAGGC1280.18866255932553133No Hit
GTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGTGCAGGC1270.18718863308080064No Hit
GTAGGTGGCGAGCGTTGTCCGGATTGACTGGGCGTAAAGGGAGCGTAGGC1230.1812929281018778No Hit
GTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGTGCAGGC1210.17834507561241636No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGCGCAGG1190.17539722312295494No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGG1190.17539722312295494No Hit
GTAGGTGGCGAGCGTTGTCCGGATGTACTGGGCGTAAAGGGAGCGTAGGC1180.17392329687822422No Hit
CGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGG1150.16950151814403208No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG1130.16655366565457064No Hit
GTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGTGCAGGC1130.16655366565457064No Hit
GTAGGATGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAGGC1120.16507973940983994No Hit
CGTAGGTGGCGAGCGTTGTCCGGAGTTACTGGGCGTAAAGGGAGCGTAGG1100.1621318869203785No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA1080.15918403443091708No Hit
GTAGGTGGCGAGCGTGGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGC1080.15918403443091708No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTAG1030.1518144032072635No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGTGCAGG990.14591869822834067No Hit
GTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGC960.1414969194941485No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGGC940.13854906700468708No Hit
CGTAGGTGGCGAGCGTTGTCGGGATTTACTGGGCGTAAAGGGAGCGTAGG930.1370751407599564No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGCGCAGG920.13560121451522564No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC880.1297055095363028No Hit
GTAGGTGGCAAGCGTTGTCGGGATTTATTGGGCGTAAAGCGAGTGCAGGC880.1297055095363028No Hit
CGTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGCGCAGG870.1282315832915721No Hit
ACGTAGGGGGCTAGCGGTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG850.12528373080211067No Hit
CGTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGTGCAGG840.12380980455737994No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGCGCAGGC820.12086195206791853No Hit
CGTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGCGCAGG810.11938802582318782No Hit
GTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGCGCAGGC780.11496624708899567No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGGC780.11496624708899567No Hit
GTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGCGCAGGC780.11496624708899567No Hit
CGTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGTGCAGG740.1090705421100728No Hit
GTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGCGCAGGC710.10464876337588068No Hit
CGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGG690.10170091088641925No Hit
ACGTAGGGGGCTAGCGTTATCGGGAATTACTGGGCGTAAAGGGTGCGTAG690.10170091088641925No Hit
CGTAGGTGGCGAGCGTTGTCCGGATTGACTGGGCGTAAAGGGAGCGTAGG690.10170091088641925No Hit
CGTAGGGGGCTAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTAGG680.10022698464168853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)