FastQCFastQC Report
Sat 26 Oct 2024
IN15-50_43_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN15-50_43_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67846
Sequences flagged as poor quality0
Sequence length248-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA67229.907732217079857No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG48467.142646581965038No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA48087.086637384665272No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGAAG12331.81735105975297No Hit
TGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG10651.56973145063821No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA8561.2616808654894909No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG8181.205671668189724No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGTAT7411.0921793473454589No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG7401.090705421100728No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA7071.0420658550246145No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC6811.0037437726616159No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG6220.9167821242225038No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG5720.8430858119859682No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG5260.7752852047283554No Hit
TGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAGAC5220.7693894997494326No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG5000.7369631223653569No Hit
CTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGAGCGCCGGTAT4220.6219968752763612No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAG3960.5836747929133627No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGAAAG3740.5512484155292869No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAG3520.5188220381452112No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAAT3290.4849217345164048No Hit
CTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCCGGCACCGGTAT3190.4701824720690977No Hit
CTGTTCGATACCCGCACTTTCGTGCCTGAGCGTCAGTTGAGCGCCGGTAA2890.4259646847271763No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAAAG2860.4215429059929841No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG2710.3994340123220234No Hit
TGTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG2490.3670076349379477No Hit
TGTTCGATACCCACACTTTCGAGCTTCAGCGTCAGTTACGATACAGTCAG2050.3021548801697963No Hit
CTGTTTGCTCCCCACGCTGTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA1960.2888895439672199No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTAT1950.28741561772248914No Hit
TGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCAGCAAG1910.2815199127435663No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGAAG1860.2741502815199128No Hit
CTGTTTGATTCCCGCACTTTCGAGCCTCAGCGTCAATCATACCCCAGGGG1810.2667806502962592No Hit
CTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCCGGAGCCGGTAA1740.2564631665831442No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGG1730.25498924033841347No Hit
TGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCGGCAAG1530.2255107154437992No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCAGG1500.22108893670960708No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC1500.22108893670960708No Hit
TGTTTGCTCCCCACGCTTTCGAGCCCCAGCGTCAGTTACAGTCCAGAGAA1480.2181410842201456No Hit
GTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGC1450.21371930548595347No Hit
CCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAAA1420.20929752675176136No Hit
CTGTGTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA1410.20782360050703064No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAAAG1380.2034018217728385No Hit
TGGTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG1350.19898004303864633No Hit
CTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAATAGCACCCCAGGAG1340.19750611679391564No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGAAAGACACCGGTAC1340.19750611679391564No Hit
TGTTCGCTACCCATGCTTCCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1340.19750611679391564No Hit
GGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC1320.1945582643044542No Hit
CTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCCGGCGCCGGTAC1190.17539722312295494No Hit
TGTTTGATACCCACACTTTCGAGCATCAATGTCAGTGGCAGTTTAGTAGG1160.17097544438876278No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT1160.17097544438876278No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAG1150.16950151814403208No Hit
TGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGTGG1130.16655366565457064No Hit
CTGGTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGTAT1110.16360581316510922No Hit
TGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA1070.15771010818618636No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGA1060.15623618194145567No Hit
CTGTTCGATCCCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTAT1040.15328832945199422No Hit
TGTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1030.1518144032072635No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTAT990.14591869822834067No Hit
GTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAGC960.1414969194941485No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAG960.1414969194941485No Hit
CTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGTAT920.13560121451522564No Hit
TGTTCGCTACCCATGCTTTCGAGCCGCAGCGTCAGTTGCAGACCAGAGAG920.13560121451522564No Hit
CTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTAT890.13117943578103353No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAG870.1282315832915721No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAG850.12528373080211067No Hit
TGTTTGCTACCCACGCTTTCGCTCCTCAGTGTCAGTTATGTGCCAGATAG830.12233587831264923No Hit
TGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAG820.12086195206791853No Hit
TGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTAAG820.12086195206791853No Hit
CGGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA820.12086195206791853No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG810.11938802582318782No Hit
CTGGTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGAGCGCCGGTAT800.11791409957845708No Hit
TGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTATG800.11791409957845708No Hit
TGTTCGCTACCCAGGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG800.11791409957845708No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTACAGACCAGGTA790.11644017333372637No Hit
CTGTTTGCTCCCCACGCGTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA790.11644017333372637No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCTCAGTCCAGCAGG780.11496624708899567No Hit
TGTTTGCTCCCCACGCGTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA750.11054446835480354No Hit
TGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTTGCAGTCCAGCAGG750.11054446835480354No Hit
TGTGTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA730.1075966158653421No Hit
GTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC730.1075966158653421No Hit
GGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAAT730.1075966158653421No Hit
GTGCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC720.10612268962061139No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG720.10612268962061139No Hit
CTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTGT680.10022698464168853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)