FastQCFastQC Report
Sat 26 Oct 2024
IN14-50_30_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN14-50_30_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61325
Sequences flagged as poor quality0
Sequence length35-251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGG4310.7028128821850794No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGC3150.5136567468406034No Hit
CGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGA2580.4207093355075418No Hit
GTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGAC2500.40766408479412963No Hit
GTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAGGC2460.4011414594374235No Hit
CGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAGG2340.38157358336730535No Hit
GTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGC2090.34080717488789236No Hit
GTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGC1920.31308601712189155No Hit
CGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGG1900.30982470444353855No Hit
GTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGC1400.22829188748471257No Hit
ACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGCAG1400.22829188748471257No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGG1290.2103546677537709No Hit
GTAGGTGGCGAGCGTTATCGGGAATGATTGGGCGTAAAGGGTGCGCAGGC1120.1826335099877701No Hit
ACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTAG1010.16469629025682836No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGG950.15491235222176927No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGG940.15328169588259274No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGG880.14349775784753363No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCC840.13697513249082757No Hit
CGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGC840.13697513249082757No Hit
GGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGCGTAGGC830.13534447615165104No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTAGTGGGCGTAAAGCGAGTGCAGGC830.13534447615165104No Hit
GTAGGTGGCAAGCGTTGTCCGGATGTATTGGGCGTAAAGCGAGTGCAGGC820.13371381981247452No Hit
GTAGGTGGCAAGCGTTGTCCGGATTGATTGGGCGTAAAGCGAGTGCAGGC810.13208316347329802No Hit
GTAGGTGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGCGAGTGCAGGC810.13208316347329802No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCAGG770.12556053811659193No Hit
CGTAGGTGGCGAGCGTTATCGGGAATGATTGGGCGTAAAGGGTGCGCAGG690.11251528740317979No Hit
GTAGGTGGCAAGCGTTGTCGGGATTTATTGGGCGTAAAGCGAGTGCAGGC680.11088463106400327No Hit
CGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCGCGTAGG680.11088463106400327No Hit
CGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGG670.10925397472482674No Hit
GTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGC650.1059926620464737No Hit
GTAGGGGGCAAGCGTTATCGGGATTTACTGGGTGTAAAGGGAGCGTAGAC640.10436200570729719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)