FastQCFastQC Report
Sat 26 Oct 2024
IN14-50_30_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIN14-50_30_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61325
Sequences flagged as poor quality0
Sequence length248-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG36735.989400733795352No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA18933.0868324500611495No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG16992.770485120260905No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG14992.4443538524256017No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT10481.7089278434569914No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGGCCAGGCG9971.625764370158989No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG9031.4724826742763963No Hit
CTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA6951.1333061557276805No Hit
TGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG6381.0403587443946187No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGA6321.0305748063595597No Hit
TGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAGAC6251.019160211985324No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAG5700.9294741133306157No Hit
TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGAG5200.8479412963717896No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTCAT4780.7794537301263759No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC4010.6538931920097839No Hit
TGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTACGGTCCAGTAAG3420.5576844679983693No Hit
TGTTTGCTCCCCGCACCTTCGCACCTCAGCGTCAGTTATCTGCTTGAAAC3380.5511618426416632No Hit
TGTTTGCTACCCACGCTTTCGCTCCTCAGTGTCAGTTATGTGCCAGATAG2870.4679983693436608No Hit
TATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCAG2730.4451691805951895No Hit
TGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG2430.396249490419894No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCTCAGTCCAGCAGG2410.39298817774154093No Hit
TGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGTGG2210.3603750509580106No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAA2130.3473298002445985No Hit
TAGTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG1900.30982470444353855No Hit
GTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGC1810.29514879739094985No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAG1790.2918874847125968No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAG1770.2886261720342438No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCAGA1650.26905829596412556No Hit
CTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGATGGCCCAGAAG1600.260905014268243No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTGGGCCAGAAAG1550.2527517325723604No Hit
TGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTTGCAGTCCAGCAGG1550.2527517325723604No Hit
TGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG1540.25112107623318386No Hit
TGGTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1460.2380758255197717No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGCAAG1420.23155320016306566No Hit
TGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAG1420.23155320016306566No Hit
CCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGATTCGGTCCGGAAA1370.22339991846718305No Hit
TGTTTGCTCCCCATGCTTTCGCGCATTAGCGTCAGTAATGTTCCAGTAAG1350.22013860578883002No Hit
TGTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG1340.2185079494496535No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTAAC1340.2185079494496535No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGAAG1290.2103546677537709No Hit
TGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCGGCAAG1250.20383204239706482No Hit
TGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGGCCAGGTGG1250.20383204239706482No Hit
CTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGAGCGCCGGTAT1230.20057072971871176No Hit
TATTTGCTCCCCACGCTTGCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG1220.19894007337953526No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTAT1170.19078679168365267No Hit
TGTTTGCTCCCCACGCTTTCGCGCATCAGCGTCAGTTATGTTCCAGCAGG1150.18752547900529964No Hit
CTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAATCGCACTCCAGGGA1110.18100285364859356No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAT1100.17937219730941703No Hit
TGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTTCCAGCCAGATTG1060.17284957195271095No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGG1060.17284957195271095No Hit
CTGGTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAGA1030.16795760293518142No Hit
CTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTGCACCCCGGGCA990.16143497757847536No Hit
CTAGTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG970.1581736649001223No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATC970.1581736649001223No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCAGG960.15654300856094577No Hit
TATTTGCGCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG960.15654300856094577No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAAG950.15491235222176927No Hit
CTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTCGGGCGCCGGTAT920.15002038320423972No Hit
CTATTTGCTCCCCACGCTGTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG890.14512841418671016No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGC870.1418671015083571No Hit
GTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAAT860.1402364451691806No Hit
TGTTCGCTACCCATGCTTCCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG860.1402364451691806No Hit
TGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGG850.13860578883000407No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAG840.13697513249082757No Hit
TGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAG830.13534447615165104No Hit
CTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAAG810.13208316347329802No Hit
TGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCAGTCCAGTAAG800.1304525071341215No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTAAG780.12719119445576843No Hit
TGGTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAGAG770.12556053811659193No Hit
TATGTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGCAACAGGCCAGGCGG770.12556053811659193No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAG760.12392988177741542No Hit
TGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTAAG750.12229922543823889No Hit
GTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGAC740.12066856909906237No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGTCCAGCAGG740.12066856909906237No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCAGG730.11903791275988584No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCAGG690.11251528740317979No Hit
TGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTTAT680.11088463106400327No Hit
TGGTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGA680.11088463106400327No Hit
GTTCGCTACCCATGCTTGCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGC660.10762331838565023No Hit
TGTTCGCTACCCAGGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAG660.10762331838565023No Hit
CTATGTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCACAGGCCAGGCG660.10762331838565023No Hit
CTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAACAGTCCAGGCG650.1059926620464737No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGCAGG630.10273134936812067No Hit
TGTTCGCTCCCCGCACCTTCGCACCTCAGCGTCAGTCTTCTGCCGGCAAC620.10110069302894414No Hit
TATTTGCTCCCCACGCTTTCGTGCCGCAGCGTCAGCAACAGGCCAGGCGG620.10110069302894414No Hit
TGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAG620.10110069302894414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)